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LRRC45 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - LRRC45 Antibody (C-term) AP11368b
    Western blot analysis of lysates from human blood plasma tissue (from left to right),using LRRC45 Antibody (C-term)(Cat. #AP11368b).AP11368b was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.
  • IF - LRRC45 Antibody (C-term) AP11368b
    Immunofluorescent analysis of U251 cells, using LRRC45 Antibody (C-term) (Cat. #AP11368b). AP11368b was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor® 554 (red) conjugated Phalloidin (red).
  • IHC-P - LRRC45 Antibody (C-term) AP11368b
    LRRC45 Antibody (C-term) (Cat. #AP11368b)immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of LRRC45 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - LRRC45 Antibody (C-term) AP11368b
    LRRC45 Antibody (C-term) (Cat. #AP11368b) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q96CN5
Other Accession NP_659436.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 75951 Da
Antigen Region 615-643 aa
Additional Information
Gene ID 201255
Other Names Leucine-rich repeat-containing protein 45, LRRC45
Target/Specificity This LRRC45 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 615-643 amino acids from the C-terminal region of human LRRC45.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsLRRC45 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name LRRC45
Function Component of the proteinaceous fiber-like linker between two centrioles, required for centrosome cohesion.
Cellular Location Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Note=Localizes to the proteinaceous linker between the proximal ends of the centrioles.
Research Areas
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Olsen, J.V., et al. Cell 127(3):635-648(2006) Gerhard, D.S., et al. Genome Res. 14 (10B), 2121-2127 (2004) :

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$ 295.00
$ 99.00
Cat# AP11368b
(40 western blots)
Availability: In Stock
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