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ATP6V1B1 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - ATP6V1B1 Antibody (Center) AP11538C
    All lanes : Anti-ATP6V1B1 Antibody (Center) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - ATP6V1B1 Antibody (Center) AP11538C
    ATP6V1B1 Antibody (Center) (Cat. #AP11538c)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ATP6V1B1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • IF - ATP6V1B1 Antibody (Center) AP11538C
    Confocal immunofluorescent analysis of ATP6V1B1 Antibody (Center)(Cat#AP11538c) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P15313
Other Accession P31409, P49712, Q19626, P62815, P62814, P21281, P31408, P31407, NP_001683.2
Reactivity Human
Predicted Bovine, Mouse, Rat, C.Elegans, Chicken, Drosophila
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 56833 Da
Antigen Region 284-310 aa
Additional Information
Gene ID 525
Other Names V-type proton ATPase subunit B, kidney isoform, V-ATPase subunit B 1, Endomembrane proton pump 58 kDa subunit, Vacuolar proton pump subunit B 1, ATP6V1B1, ATP6B1, VATB, VPP3
Target/Specificity This ATP6V1B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 284-310 amino acids from the Central region of human ATP6V1B1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsATP6V1B1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATP6V1B1
Synonyms ATP6B1, VATB, VPP3
Function Non-catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.
Cellular Location Endomembrane system; Peripheral membrane protein. Note=Endomembrane
Tissue Location Expressed in the cochlea and endolymphatic sac.
Research Areas
Citations (0)

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This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain B subunit isoforms and is found in the kidney. Mutations in this gene cause distal renal tubular acidosis associated with sensorineural deafness. [provided by RefSeq].


Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Sharifian, M., et al. Iran J Kidney Dis 4(3):202-206(2010)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Andreucci, E., et al. Pediatr. Nephrol. 24(11):2147-2153(2009)
Sethi, S.K., et al. Indian Pediatr 46(5):425-427(2009)

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$ 295.00
$ 99.00
Cat# AP11538C
(40 western blots)
Availability: In Stock
Bulk Size
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