|Application ||WB, E|
|Other Accession||P09838, NP_001017520.1, NP_004079.3|
|Calculated MW||58536 Da|
|Antigen Region||438-467 aa|
|Other Names||DNA nucleotidylexotransferase, Terminal addition enzyme, Terminal deoxynucleotidyltransferase, Terminal transferase, DNTT, TDT|
|Target/Specificity||This TdT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 438-467 amino acids from the C-terminal region of human TdT.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TdT Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.|
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Provided below are standard protocols that you may find useful for product applications.
This gene is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described.
Kubota, T., et al. Genes Cells 12(8):941-959(2007)
O'Malley, D.P., et al. Haematologica 91(8):1139-1140(2006)
Grupe, A., et al. Am. J. Hum. Genet. 78(1):78-88(2006)
Thai, T.H., et al. J. Immunol. 173(6):4009-4019(2004)
Liu, L., et al. Am. J. Clin. Pathol. 121(6):810-815(2004)
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