|Application ||WB, IHC-P, IF, E|
|Other Accession||Q64331, NP_004990.3|
|Calculated MW||149691 Da|
|Antigen Region||1166-1195 aa|
|Other Names||Unconventional myosin-VI, Unconventional myosin-6, MYO6, KIAA0389|
|Target/Specificity||This MYO6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1166-1195 amino acids from the C-terminal region of human MYO6.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MYO6 Antibody (C-term R1181) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Myosin 6 is a reverse-direction motor protein that moves towards the minus-end of actin filaments. Has slow rate of actin-activated ADP release due to weak ATP binding. Functions in a variety of intracellular processes such as vesicular membrane trafficking and cell migration. Required for the structural integrity of the Golgi apparatus via the p53-dependent pro-survival pathway. Appears to be involved in a very early step of clathrin-mediated endocytosis in polarized epithelial cells. May act as a regulator of F-actin dynamics. May play a role in transporting DAB2 from the plasma membrane to specific cellular targets. Required for structural integrity of inner ear hair cells (By similarity).|
|Cellular Location||Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Golgi apparatus. Nucleus. Cytoplasm, perinuclear region. Membrane, clathrin-coated pit. Cell projection, ruffle membrane; Peripheral membrane protein. Note=Also present in endocyctic vesicles, and membrane ruffles. Translocates from membrane ruffles, endocytic vesicles and cytoplasm to Golgi apparatus, perinuclear membrane and nucleus through induction by p53 and p53-induced DNA damage Recruited into membrane ruffles from cell surface by EGF- stimulation. Colocalizes with DAB2 in clathrin-coated pits/vesicles. Colocalizes with OPTN at the Golgi complex and in vesicular structures close to the plasma membrane (By similarity) Isoform 4: Cytoplasmic vesicle, clathrin- coated vesicle membrane. Cell projection, ruffle membrane|
|Tissue Location||Expressed in most tissues examined including heart, brain, placenta, pancreas, spleen, thymus, prostate, testis, ovary, small intestine and colon. Highest levels in brain, pancreas, testis and small intestine. Also expressed in fetal brain and cochlea. Isoform 1 and isoform 2, containing the small insert, and isoform 4, containing neither insert, are expressed in unpolarized epithelial cells.|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a protein involved intracellular vesicle and organelle transport, especially in the hair cell of the inner ear. Mutations in this gene have been found in patients with non-syndromic autosomal dominant and recessive hearing loss.
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Nishikawa, S., et al. Cell 142(6):879-888(2010)
Cho, S.J., et al. J. Biol. Chem. 285(35):27159-27166(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Szczyrba, J., et al. Mol. Cancer Res. 8(4):529-538(2010)
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