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ADA Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - ADA Antibody (C-term) AP11650b
    ADA Antibody (C-term) (Cat. #AP11650b) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the ADA antibody detected the ADA protein (arrow).
  • FC - ADA Antibody (C-term) AP11650b
    ADA Antibody (C-term) (Cat. #AP11650b) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P00813
Other Accession NP_000013.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 40764 Da
Antigen Region 287-314 aa
Additional Information
Gene ID 100
Other Names Adenosine deaminase, Adenosine aminohydrolase, ADA, ADA1
Target/Specificity This ADA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 287-314 amino acids from the C-terminal region of human ADA.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsADA Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ADA
Synonyms ADA1
Function Catalyzes the hydrolytic deamination of adenosine and 2- deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte- epithelial cell adhesion.
Cellular Location Cell membrane; Peripheral membrane protein; Extracellular side. Cell junction. Cytoplasmic vesicle lumen. Cytoplasm. Note=Colocalized with DPP4 at the cell junction in lymphocyte-epithelial cell adhesion
Tissue Location Found in all tissues, occurs in large amounts in T-lymphocytes and, at the time of weaning, in gastrointestinal tissues
Research Areas
Citations (0)

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This gene encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine. Various mutations have been described for this gene and have been linked to human diseases. Deficiency in this enzyme causes a form of severe combined immunodeficiency disease (SCID), in which there is dysfunction of both B and T lymphocytes with impaired cellular immunity and decreased production of immunoglobulins, whereas elevated levels of this enzyme have been associated with congenital hemolytic anemia.


Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Gloria-Bottini, F., et al. Am. J. Med. Sci. 340(2):103-108(2010)
Levine, A.J., et al. Cancer Epidemiol. Biomarkers Prev. 19(7):1812-1821(2010)
Spina, C., et al. Cancer Invest. (2010) In press :
Ri, G., et al. Anticancer Res. 30(6):2347-2349(2010)

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$ 295.00
$ 99.00
Cat# AP11650b
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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