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ADA Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - ADA Antibody (C-term) AP11650b
    All lanes : Anti-ADA Antibody (C-term) at 1:1000 dilution Lane 1: MOLT-4 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: Hela whole cell lysate Lane 4: HL-60 whole cell lysate Lane 5: DU145 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 41 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - ADA Antibody (C-term) AP11650b
    ADA Antibody (C-term) (Cat. #AP11650b) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the ADA antibody detected the ADA protein (arrow).
  • FC - ADA Antibody (C-term) AP11650b
    ADA Antibody (C-term) (Cat. #AP11650b) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession P00813
Other Accession NP_000013.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 40764 Da
Antigen Region 287-314 aa
Additional Information
Gene ID 100
Other Names Adenosine deaminase, Adenosine aminohydrolase, ADA, ADA1
Target/Specificity This ADA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 287-314 amino acids from the C-terminal region of human ADA.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsADA Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ADA
Synonyms ADA1
Function Catalyzes the hydrolytic deamination of adenosine and 2- deoxyadenosine (PubMed:8452534, PubMed:16670267). Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4 (PubMed:20959412). Its interaction with DPP4 regulates lymphocyte- epithelial cell adhesion (PubMed:11772392). Enhances dendritic cell immunogenicity by affecting dendritic cell costimulatory molecule expression and cytokines and chemokines secretion (By similarity). Enhances CD4+ T-cell differentiation and proliferation (PubMed:20959412). Acts as a positive modulator of adenosine receptors ADORA1 and ADORA2A, by enhancing their ligand affinity via conformational change (PubMed:23193172). Stimulates plasminogen activation (PubMed:15016824). Plays a role in male fertility (PubMed:21919946, PubMed:26166670). Plays a protective role in early postimplantation embryonic development (By similarity).
Cellular Location Cell membrane; Peripheral membrane protein; Extracellular side. Cell junction Cytoplasmic vesicle lumen {ECO:0000250|UniProtKB:P03958} Cytoplasm. Lysosome Note=Colocalized with DPP4 at the cell surface
Tissue Location Found in all tissues, occurs in large amounts in T-lymphocytes (PubMed:20959412). Expressed at the time of weaning in gastrointestinal tissues
Research Areas
Citations (0)

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Background

This gene encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine. Various mutations have been described for this gene and have been linked to human diseases. Deficiency in this enzyme causes a form of severe combined immunodeficiency disease (SCID), in which there is dysfunction of both B and T lymphocytes with impaired cellular immunity and decreased production of immunoglobulins, whereas elevated levels of this enzyme have been associated with congenital hemolytic anemia.

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Gloria-Bottini, F., et al. Am. J. Med. Sci. 340(2):103-108(2010)
Levine, A.J., et al. Cancer Epidemiol. Biomarkers Prev. 19(7):1812-1821(2010)
Spina, C., et al. Cancer Invest. (2010) In press :
Ri, G., et al. Anticancer Res. 30(6):2347-2349(2010)

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$ 295.00
$ 99.00
Cat# AP11650b
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Quantity:
(40 western blots)
Availability: In Stock
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