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SSH3 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - SSH3 Antibody (C-term) AP11697b
    Anti-SSH3 Antibody (C-term) at 1:1000 dilution + ZR-75-1 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 73 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - SSH3 Antibody (C-term) AP11697b
    SSH3 Antibody (C-term) (Cat. #AP11697b)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SSH3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - SSH3 Antibody (C-term) AP11697b
    SSH3 Antibody (C-term) (Cat. #AP11697b) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • IF - SSH3 Antibody (C-term) AP11697b
    Confocal immunofluorescent analysis of SSH3 Antibody (C-term)(Cat#AP11697b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8TE77
Other Accession NP_060327.3
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 72996 Da
Antigen Region 575-602 aa
Additional Information
Gene ID 54961
Other Names Protein phosphatase Slingshot homolog 3, SSH-like protein 3, SSH-3L, hSSH-3L, SSH3, SSH3L
Target/Specificity This SSH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 575-602 amino acids from the C-terminal region of human SSH3.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSSH3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SSH3
Synonyms SSH3L
Function Protein phosphatase which may play a role in the regulation of actin filament dynamics. Can dephosphorylate and activate the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly (By similarity).
Cellular Location Cytoplasm, cytoskeleton. Nucleus
Research Areas
Citations (0)

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The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin dynamics. ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1; MIM 601329). The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo (Niwa et al., 2002 [PubMed 11832213]).


Kousaka, K., et al. Genesis 46(5):246-255(2008)
Kligys, K., et al. J. Biol. Chem. 282(44):32520-32528(2007)
Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006)
Soosairajah, J., et al. EMBO J. 24(3):473-486(2005)
Ohta, Y., et al. Genes Cells 8(10):811-824(2003)

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$ 295.00
$ 99.00
Cat# AP11697b
(40 western blots)
Availability: In Stock
Bulk Size
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