|Application ||WB, IHC-P, FC, IF, E|
|Calculated MW||72996 Da|
|Antigen Region||575-602 aa|
|Other Names||Protein phosphatase Slingshot homolog 3, SSH-like protein 3, SSH-3L, hSSH-3L, SSH3, SSH3L|
|Target/Specificity||This SSH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 575-602 amino acids from the C-terminal region of human SSH3.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SSH3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Protein phosphatase which may play a role in the regulation of actin filament dynamics. Can dephosphorylate and activate the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly (By similarity).|
|Cellular Location||Cytoplasm, cytoskeleton. Nucleus|
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Provided below are standard protocols that you may find useful for product applications.
The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin dynamics. ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1; MIM 601329). The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo (Niwa et al., 2002 [PubMed 11832213]).
Kousaka, K., et al. Genesis 46(5):246-255(2008)
Kligys, K., et al. J. Biol. Chem. 282(44):32520-32528(2007)
Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006)
Soosairajah, J., et al. EMBO J. 24(3):473-486(2005)
Ohta, Y., et al. Genes Cells 8(10):811-824(2003)
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