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PLEKHA4 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PLEKHA4 Antibody (N-term) AP11773a
    PLEKHA4 Antibody (N-term) (Cat. #AP11773a) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PLEKHA4 antibody detected the PLEKHA4 protein (arrow).
    detail
  • IHC-P - PLEKHA4 Antibody (N-term) AP11773a
    PLEKHA4 Antibody (N-term) (Cat. #AP11773a)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PLEKHA4 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - PLEKHA4 Antibody (N-term) AP11773a
    PLEKHA4 Antibody (N-term) (Cat. #AP11773a) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, FC, WB, E
Primary Accession Q9H4M7
Other Accession NP_065955.2, NP_001154826.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 85401 Da
Antigen Region 21-50 aa
Additional Information
Gene ID 57664
Other Names Pleckstrin homology domain-containing family A member 4, PH domain-containing family A member 4, Phosphoinositol 3-phosphate-binding protein 1, PEPP-1, PLEKHA4, PEPP1
Target/Specificity This PLEKHA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-50 amino acids from the N-terminal region of human PLEKHA4.
Dilution WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPLEKHA4 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PLEKHA4
Synonyms PEPP1
Function Binds specifically to phosphatidylinositol 3-phosphate (PtdIns3P), but not to other phosphoinositides.
Cellular Location Cytoplasm. Membrane; Peripheral membrane protein
Tissue Location Highly expressed in melanoma. Detected at low levels in heart, skeletal muscle, kidney, liver and small intestine
Research Areas
Citations (0)
citation

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Background

PLEKHA4 binds specifically to phosphatidylinositol-3-phosphate (PtdIns3P), but not to other phosphoinositides.

References

Stelzl, U., et al. Cell 122(6):957-968(2005)
Dowler, S., et al. Biochem. J. 351 (PT 1), 19-31 (2000) :

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$ 365.00
$ 140.00
Cat# AP11773a
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