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p21WAF1 Antibody (Center H450)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | O60294 |
| Other Accession | NP_055608.2 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 75602 Da |
| Antigen Region | 435-463 aa |
| Gene ID | 9836 |
|---|---|
| Other Names | tRNA wybutosine-synthesizing protein 4, tRNA yW-synthesizing protein 4, Leucine carboxyl methyltransferase 2, tRNA(Phe) (7-(3-amino-3-(methoxycarbonyl)propyl)wyosine(37)-N)-methoxycarbonyltransferase, tRNA(Phe) (7-(3-amino-3-carboxypropyl)wyosine(37)-O)-methyltransferase, LCMT2, KIAA0547, TYW4 |
| Target/Specificity | This p21WAF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 435-463 amino acids from the Central region of human p21WAF1. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | p21WAF1 Antibody (Center H450) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | LCMT2 |
|---|---|
| Synonyms | KIAA0547, TYW4 |
| Function | Probable S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA (By similarity). May methylate the carboxyl group of leucine residues to form alpha-leucine ester residues. |
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Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded by this intronless gene belongs to the highly variable methyltransferase superfamily. This gene is the inferred homolog of the Saccharomyces cerevisiae carboxymethyltransferase gene PPM2 that is essential for the synthesis of the hypermodified guanosine Wybutosine (yW). [provided by RefSeq].
References
Noma, A., et al. EMBO J. 25(10):2142-2154(2006)
De Baere, I., et al. Biochemistry 38(50):16539-16547(1999)
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