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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   p21WAF1 Antibody (Center H450)   

p21WAF1 Antibody (Center H450)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - p21WAF1 Antibody (Center H450) AP11778c
    p21WAF1 Antibody (Center H450) (Cat. #AP11778c) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the p21WAF1 antibody detected the p21WAF1 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O60294
Other Accession NP_055608.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 75602 Da
Antigen Region 435-463 aa
Additional Information
Gene ID 9836
Other Names tRNA wybutosine-synthesizing protein 4, tRNA yW-synthesizing protein 4, Leucine carboxyl methyltransferase 2, tRNA(Phe) (7-(3-amino-3-(methoxycarbonyl)propyl)wyosine(37)-N)-methoxycarbonyltransferase, tRNA(Phe) (7-(3-amino-3-carboxypropyl)wyosine(37)-O)-methyltransferase, LCMT2, KIAA0547, TYW4
Target/Specificity This p21WAF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 435-463 amino acids from the Central region of human p21WAF1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautionsp21WAF1 Antibody (Center H450) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name LCMT2
Synonyms KIAA0547, TYW4
Function Probable S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA (By similarity). May methylate the carboxyl group of leucine residues to form alpha- leucine ester residues.
Research Areas
Citations (0)

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The protein encoded by this intronless gene belongs to the highly variable methyltransferase superfamily. This gene is the inferred homolog of the Saccharomyces cerevisiae carboxymethyltransferase gene PPM2 that is essential for the synthesis of the hypermodified guanosine Wybutosine (yW). [provided by RefSeq].


Noma, A., et al. EMBO J. 25(10):2142-2154(2006)
De Baere, I., et al. Biochemistry 38(50):16539-16547(1999)

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$ 295.00
$ 99.00
Cat# AP11778c
(40 western blots)
Availability: In Stock
Bulk Size
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