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LRWD1 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | Q9UFC0 |
| Other Accession | NP_690852.1 |
| Reactivity | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 70861 Da |
| Antigen Region | 1-30 aa |
| Gene ID | 222229 |
|---|---|
| Other Names | Leucine-rich repeat and WD repeat-containing protein 1, Centromere protein 33, CENP-33, Origin recognition complex-associated protein, ORC-associated protein, ORCA, LRWD1, CENP33, ORCA |
| Target/Specificity | This LRWD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human LRWD1. |
| Dilution | WB~~1:1000 IHC-P~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | LRWD1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | LRWD1 |
|---|---|
| Synonyms | CENP-33 {ECO:0000303|PubMed:20813266}, O |
| Function | Required for G1/S transition. Recruits and stabilizes the origin recognition complex (ORC) onto chromatin during G1 to establish pre-replication complex (preRC) and to heterochromatic sites in post- replicated cells. Binds a combination of DNA and histone methylation repressive marks on heterochromatin. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3 in a cooperative manner with DNA methylation. Required for silencing of major satellite repeats. May be important ORC2, ORC3 and ORC4 stability. |
| Cellular Location | Nucleus. Chromosome, centromere. Chromosome, telomere. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000250|UniProtKB:Q8BUI3}. Chromosome, centromere, kinetochore. Note=Localizes to heterochromatin during G1 phase. Restricted to centromeres or telomeres as cells progress though S phase. When cells enter mitosis, relocalizes to centromeres Recruitment to pericentric heterochromatin largely depends on the presence of H3K9me3 |
| Tissue Location | Testis-specific. Drastically down-regulated in testis from patients with Sertoli cell-only syndrome (SCOS) |
Research Areas
Citations ( 0 )
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
Background
LRWD1, Leucine rich repeats and WD repeat domain containing 1, contains 3 LRR (leucine-rich) repeats and 5 WD repeats.
References
Teng, Y.N., et al. Int. J. Androl. 33(6):832-840(2010)
Shen, Z., et al. Mol. Cell 40(1):99-111(2010)
Vermeulen, M., et al. Cell 142(6):967-980(2010)
Matsuoka, S., et al. Science 316(5828):1160-1166(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
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$ 182.50
$ 70.00
Cat# AP11807a
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