|Application ||WB, IHC-P, E|
|Calculated MW||70861 Da|
|Antigen Region||1-30 aa|
|Other Names||Leucine-rich repeat and WD repeat-containing protein 1, Centromere protein 33, CENP-33, Origin recognition complex-associated protein, ORC-associated protein, ORCA, LRWD1, CENP33, ORCA|
|Target/Specificity||This LRWD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human LRWD1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||LRWD1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Required for G1/S transition. Recruits and stabilizes the origin recognition complex (ORC) onto chromatin during G1 to establish pre-replication complex (preRC) and to heterochromatic sites in post-replicated cells. Binds a combination of DNA and histone methylation repressive marks on heterochromatin. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K20me3 and H4K27me3 in a cooperative manner with DNA methylation. Required for silencing of major satellite repeats. May be important ORC2, ORC3 and ORC4 stability.|
|Cellular Location||Nucleus. Chromosome, centromere. Chromosome, telomere. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Localizes to heterochromatin during G1 phase. Restricted to centromeres or telomeres as cells progress though S phase. When cells enter mitosis, relocalizes to centromeres. Recruitment to pericentric heterochromatin largely depends on the presence of H3K9me3|
|Tissue Location||Testis-specific. Drastically down-regulated in testis from patients with Sertoli cell-only syndrome (SCOS)|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
LRWD1, Leucine rich repeats and WD repeat domain containing 1, contains 3 LRR (leucine-rich) repeats and 5 WD repeats.
Teng, Y.N., et al. Int. J. Androl. 33(6):832-840(2010)
Shen, Z., et al. Mol. Cell 40(1):99-111(2010)
Vermeulen, M., et al. Cell 142(6):967-980(2010)
Matsuoka, S., et al. Science 316(5828):1160-1166(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
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