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ROPN1L Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ROPN1L Antibody (N-term) AP11844a
    ROPN1L Antibody (N-term) (Cat. #AP11844a) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the ROPN1L antibody detected the ROPN1L protein (arrow).
    detail
  • IHC-P - ROPN1L Antibody (N-term) AP11844a
    ROPN1L Antibody (N-term) (Cat. #AP11844a)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ROPN1L Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession Q96C74
Other Accession Q9EQ00, Q4R760, Q3T024, NP_114122.2
Reactivity Mouse
Predicted Bovine, Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 26107 Da
Antigen Region 32-60 aa
Additional Information
Gene ID 83853
Other Names Ropporin-1-like protein, ROPN1-like protein, AKAP-associated sperm protein, ROPN1L, ASP
Target/Specificity This ROPN1L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-60 amino acids from the N-terminal region of human ROPN1L.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsROPN1L Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ROPN1L
Synonyms ASP, RSPH11 {ECO:0000303|PubMed:27120127
Function Functions as part of axonemal radial spoke complexes that play an important part in the motility of sperm and cilia. Important for male fertility. With ROPN1, involved in fibrous sheath integrity and sperm motility, plays a role in PKA-dependent signaling processes required for spermatozoa capacitation.
Cellular Location Cell projection, cilium, flagellum {ECO:0000250|UniProtKB:Q9EQ00}. Cell projection, cilium
Research Areas
Citations (0)
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Background

The protein encoded by this gene is a sperm protein, which interacts with A-kinase anchoring protein, AKAP3, through the amphipathic helix region of AKAP3. Type II regulatory subunit of cAMP-dependent protein kinase (PKARII) also binds to this helix domain of AKAP3, which allows PKARII to be targeted to specific subcellular compartments. It is suggested that sperm contains several proteins that bind to AKAPs in a manner similar to PKARII, and this encoded protein may be one of them.

References

Carr, D.W., et al. J. Biol. Chem. 276(20):17332-17338(2001)

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$ 182.50
$ 70.00
Cat# AP11844a
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