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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   PRKAG1 Antibody (N-term)   

PRKAG1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - PRKAG1 Antibody (N-term) AP11853a
    PRKAG1 Antibody (N-term) (Cat. #AP11853a) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the PRKAG1 antibody detected the PRKAG1 protein (arrow).
  • WB - PRKAG1 Antibody (N-term) AP11853a
    PRKAG1 Antibody (N-term) (Cat. #AP11853a) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the PRKAG1 antibody detected the PRKAG1 protein (arrow).
  • WB - PRKAG1 Antibody (N-term) AP11853a
    Western blot analysis of PRKAG1 (arrow) using rabbit polyclonal PRKAG1 Antibody (N-term) (Cat. #AP11853a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PRKAG1 gene.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P54619
Other Accession NP_002724.1, NP_997626.1
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 37579 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 5571
Other Names 5'-AMP-activated protein kinase subunit gamma-1, AMPK gamma1, AMPK subunit gamma-1, AMPKg, PRKAG1
Target/Specificity This PRKAG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PRKAG1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPRKAG1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive.
Research Areas
Citations (0)

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The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed.


Jablonski, K.A., et al. Diabetes 59(10):2672-2681(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Jassim, G., et al. Pharmacopsychiatry (2010) In press :
Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010)
McGeachie, M., et al. Circulation 120(24):2448-2454(2009)

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$ 295.00
$ 99.00
Cat# AP11853a
(40 western blots)
Availability: In Stock
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