Register or Login
All
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   GAS1 Antibody (N-term)   

GAS1 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - GAS1 Antibody (N-term) AP11869a
    GAS1 Antibody (N-term) (Cat. #AP11869a) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the GAS1 antibody detected the GAS1 protein (arrow).
  • IHC-P - GAS1 Antibody (N-term) AP11869a
    GAS1 Antibody (N-term) (Cat. #AP11869a)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GAS1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • IF - GAS1 Antibody (N-term) AP11869a
    Confocal immunofluorescent analysis of GAS1 Antibody (N-term) (Cat. #AP11869a) with NCI-H460 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
  • FC - GAS1 Antibody (N-term) AP11869a
    GAS1 Antibody (N-term) (Cat. #AP11869a) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • SPECIFICATION
  • CITATIONS: 1
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, FC, E
Primary Accession P54826
Other Accession Q01721, NP_002039
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Antigen Region 95-127 aa
Additional Information
Other Names Growth arrest-specific protein 1, GAS-1, GAS1
Target/Specificity This GAS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-127 amino acids from the N-terminal region of human GAS1.
Dilution WB~~1:1000
IHC-P~~1:50~100
IF~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGAS1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GAS1
Function Specific growth arrest protein involved in growth suppression. Blocks entry to S phase. Prevents cycling of normal and transformed cells.
Cellular Location Cell membrane; Lipid-anchor, GPI-anchor EMBL; L13698; AAA72368.1; -; mRNA EMBL; AL158149; CAH71308.1; -; Genomic_DNA EMBL; BC074908; AAH74908.1; -; mRNA EMBL; BC074809; AAH74809.1; -; mRNA EMBL; BC132682; AAI32683.1; -; mRNA EMBL; BC136586; AAI36587.1; -; mRNA CCDS; CCDS6674.1; - PIR; A53138; A53138 RefSeq; NP_002039.2; NM_002048.2 UniGene; Hs.65029; - ProteinModelPortal; P54826; - BioGrid; 108889; 2 STRING; 9606.ENSP00000298743; - iPTMnet; P54826; - PhosphoSitePlus; P54826; - BioMuta; GAS1; - DMDM; 218512049; - MaxQB; P54826; - PaxDb; P54826; - PeptideAtlas; P54826; - PRIDE; P54826; - Ensembl; ENST00000298743; ENSP00000298743; ENSG00000180447 GeneID; 2619; - KEGG; hsa:2619; - UCSC; uc004aox.5; human CTD; 2619; - DisGeNET; 2619; - GeneCards; GAS1; - H-InvDB; HIX0034867; - HGNC; HGNC:4165; GAS1 HPA; CAB024164; - HPA; HPA066902; - MalaCards; GAS1; - MIM; 139185; gene neXtProt; NX_P54826; - OpenTargets; ENSG00000180447; - Orphanet; 93925; Alobar holoprosencephaly Orphanet; 93924; Lobar holoprosencephaly Orphanet; 280200; Microform holoprosencephaly Orphanet; 93926; Midline interhemispheric variant of holoprosencephaly Orphanet; 220386; Semilobar holoprosencephaly Orphanet; 280195; Septopreoptic holoprosencephaly PharmGKB; PA28578; - eggNOG; ENOG410IW52; Eukaryota eggNOG; ENOG4111JS0; LUCA GeneTree; ENSGT00390000001195; - HOGENOM; HOG000233688; - HOVERGEN; HBG005804; - InParanoid; P54826; - KO; K06232; - OMA; CWQALLQ; - OrthoDB; EOG091G0MBW; - PhylomeDB; P54826; - TreeFam; TF329660; - BioCyc; ZFISH:ENSG00000180447-MONOMER; - Reactome; R-HSA-5632681; Ligand-receptor interactions Reactome; R-HSA-5635838; Activation of SMO SignaLink; P54826; - GeneWiki; GAS1; - GenomeRNAi; 2619; - PRO; PR:P54826; - Proteomes; UP000005640; Chromosome 9 Bgee; ENSG00000180447; - CleanEx; HS_GAS1; - Genevisible; P54826; HS GO; GO:0046658; C:anchored component of plasma membrane; ISS:UniProtKB GO; GO:0016021; C:integral component of membrane; NAS:UniProtKB GO; GO:0005886; C:plasma membrane; IDA:UniProtKB GO; GO:0007050; P:cell cycle arrest; IEA:UniProtKB-KW GO; GO:0045165; P:cell fate commitment; ISS:UniProtKB GO; GO:0035924; P:cellular response to vascular endothelial growth factor stimulus; IEP:UniProtKB GO; GO:0048589; P:developmental growth; ISS:UniProtKB GO; GO:0045930; P:negative regulation of mitotic cell cycle; IDA:UniProtKB GO; GO:0010955; P:negative regulation of protein processing; IMP:UniProtKB GO; GO:0042981; P:regulation of apoptotic process; ISS:UniProtKB GO; GO:0060628; P:regulation of ER to Golgi vesicle-mediated transport; IMP:UniProtKB GO; GO:0008589; P:regulation of smoothened signaling pathway; ISS:UniProtKB InterPro; IPR016017; GDNF/GAS1 Pfam; PF02351; GDNF; 1 SMART; SM00907; GDNF; 2 2: Evidence at transcript level; Cell cycle; Cell membrane; Complete proteome; Glycoprotein; GPI-anchor; Growth arrest; Lipoprotein; Membrane; Reference proteome; Signal SIGNAL 1 39 CHAIN 40 318 Growth arrest-specific protein 1 /FTId=PRO_0000021318 PROPEP 319 345 Removed in mature form. /FTId=PRO_0000021319 COMPBIAS 87 91 Poly-Ala COMPBIAS 285 288 Poly-Asp COMPBIAS 319 322 Poly-Gly COMPBIAS 337 341 Poly-Leu LIPID 318 318 GPI-anchor amidated serine CARBOHYD 117 117 N-linked (GlcNAc...). CONFLICT 216 216 A -> V (in Ref. 1; AAA72368) SEQUENCE 345 AA; 35693 MW; 31A0CBEEC3632F82 CRC64; MVAALLGGGG EARGGTVPGA WLCLMALLQL LGSAPRGSGL AHGRRLICWQ ALLQCQGEPE CSYAYNQYAE ACAPVLAQHG GGDAPGAAAA AFPASAASFS SRWRCPSHCI SALIQLNHTR RGPALEDCDC AQDENCKSTK RAIEPCLPRT SGGGAGGPGA GGVMGCTEAR RRCDRDSRCN LALSRYLTYC GKVFNGLRCT DECRTVIEDM LAMPKAALLN DCVCDGLERP ICESVKENMA RLCFGAELGN GPGSSGSDGG LDDYYDEDYD DEQRTGGAGG EQPLDDDDGV PHPPRPGSGA AASGGRGDLP YGPGRRSSGG GGRLAPRGAW TPLASILLLL LGPLF
Research Areas
Citations ( 0 )

Background

Growth arrest-specific 1 plays a role in growth suppression. GAS1 blocks entry to S phase and prevents cycling of normal and transformed cells. Gas1 is a putative tumor suppressor gene.

References

Ribeiro, L.A., et al. Am. J. Med. Genet. A 152A (7), 1688-1694 (2010) : Johnatty, S.E., et al. PLoS Genet. 6 (7), E1001016 (2010) : Rittie, L., et al. Aging Cell 8(6):738-751(2009) Zhao, L., et al. J. Biol. Chem. 284(39):26273-26285(2009) Dominguez-Monzon, G., et al. Int. J. Dev. Neurosci. 27(4):305-313(2009)

FeedBack
Abgent welcomes feedback from its customers.

If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at tech@abgent.com.

$ 295.00
$ 99.00
Cat# AP11869a
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
Request Quote Here

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanKoreaLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamOthers
Abgent, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609
USA Headquarters
(888) 735-7227 / (858) 622-0099 or (858) 875-1900

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
Crown Flash17-30% Off
Terms & Conditions