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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   PELP1 Antibody (Center R759)   

PELP1 Antibody (Center R759)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • IHC-P - PELP1 Antibody (Center R759) AP11872c
    AP11872C staining PELP1 in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • IHC-P - PELP1 Antibody (Center R759) AP11872c
    AP11872C staining PELP1 in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • WB - PELP1 Antibody (Center R759) AP11872c
    All lanes : Anti-PELP1 Antibody (Center R759) at 1:1000 dilution Lane 1: T47D whole cell lysate Lane 2: mouse testis lysate Lane 3: Hela whole cell lysate Lane 4: MCF-7 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lane 6: SW620 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 120 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - PELP1 Antibody (Center R759) AP11872c
    PELP1 Antibody (Center R759) (Cat. #AP11872c) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the PELP1 antibody detected the PELP1 protein (arrow).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q8IZL8
Other Accession Q56B11, Q9DBD5, NP_055204
Reactivity Human, Mouse
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 119700 Da
Antigen Region 744-771 aa
Additional Information
Gene ID 27043
Other Names Proline-, glutamic acid- and leucine-rich protein 1, Modulator of non-genomic activity of estrogen receptor, Transcription factor HMX3, PELP1, HMX3, MNAR
Target/Specificity This PELP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 744-771 amino acids from the Central region of human PELP1.
Dilution IHC-P~~1:25
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPELP1 Antibody (Center R759) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PELP1
Synonyms HMX3, MNAR
Function Coactivator of estrogen receptor-mediated transcription and a corepressor of other nuclear hormone receptors and sequence- specific transcription factors. Plays a role in estrogen receptor (ER) genomic activity when present in the nuclear compartment by activating the ER target genes in a hormonal stimulation dependent manner. Can facilitate ER non-genomic signaling via SRC and PI3K interaction in the cytosol. Plays a role in E2-mediated cell cycle progression by interacting with RB1. May have important functional implications in ER/growth factor cross-talk. Interacts with several growth factor signaling components including EGFR and HRS. Involved in nuclear receptor signaling via its interaction with AR and NR3C1. May promote tumorigenesis via its interaction with and modulation of several oncogenes including SRC, PI3K, STAT3 and EGFR. Plays a role in cancer cell metastasis via its ability to modulate E2-mediated cytoskeleton changes and cell migration via its interaction with SRC and PI3K. Functions as the key stabilizing component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes.
Cellular Location Nucleus, nucleoplasm. Nucleus. Cytoplasm. Note=Mainly found in the nucleoplasm, with low levels detected in the cytoplasm (By similarity). Also found associated with the plasma membrane. Mainly in cytoplasm in a subset of breast tumors. Localization is widely deregulated in endometrial cancers with predominantly cytoplasm localization in high-grade endometrial tumors.
Tissue Location Widely expressed.
Research Areas
Citations (0)

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Background

PELP1 is a coactivator of estrogen receptor (see ESR1; MIM 133430)-mediated transcription and a corepressor of other nuclear hormone receptors and sequence-specific transcription factors (Choi et al., 2004 [PubMed 15456770]).

References

Nair, S.S., et al. EMBO Rep. 11(6):438-444(2010)
Habashy, H.O., et al. Breast Cancer Res. Treat. 120(3):603-612(2010)
Vadlamudi, R.K., et al. J. Steroid Biochem. Mol. Biol. 118 (4-5), 211-218 (2010) :
Tzelepi, V., et al. Virchows Arch. 454(1):41-53(2009)
Grivas, P.D., et al. Cell. Oncol. 31(3):235-247(2009)

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$ 295.00
$ 99.00
Cat# AP11872c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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