|Application ||WB, IHC-P, E|
|Other Accession||Q56B11, Q9DBD5, NP_055204|
|Calculated MW||119700 Da|
|Antigen Region||744-771 aa|
|Other Names||Proline-, glutamic acid- and leucine-rich protein 1, Modulator of non-genomic activity of estrogen receptor, Transcription factor HMX3, PELP1, HMX3, MNAR|
|Target/Specificity||This PELP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 744-771 amino acids from the Central region of human PELP1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PELP1 Antibody (Center R759) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Coactivator of estrogen receptor-mediated transcription and a corepressor of other nuclear hormone receptors and sequence- specific transcription factors. Plays a role in estrogen receptor (ER) genomic activity when present in the nuclear compartment by activating the ER target genes in a hormonal stimulation dependent manner. Can facilitate ER non-genomic signaling via SRC and PI3K interaction in the cytosol. Plays a role in E2-mediated cell cycle progression by interacting with RB1. May have important functional implications in ER/growth factor cross-talk. Interacts with several growth factor signaling components including EGFR and HRS. Involved in nuclear receptor signaling via its interaction with AR and NR3C1. May promote tumorigenesis via its interaction with and modulation of several oncogenes including SRC, PI3K, STAT3 and EGFR. Plays a role in cancer cell metastasis via its ability to modulate E2-mediated cytoskeleton changes and cell migration via its interaction with SRC and PI3K. Functions as the key stabilizing component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes.|
|Cellular Location||Nucleus, nucleoplasm. Nucleus. Cytoplasm. Note=Mainly found in the nucleoplasm, with low levels detected in the cytoplasm (By similarity). Also found associated with the plasma membrane. Mainly in cytoplasm in a subset of breast tumors. Localization is widely deregulated in endometrial cancers with predominantly cytoplasm localization in high-grade endometrial tumors.|
|Tissue Location||Isoform 2 is expressed in breast cancer cell lines. Isoform 1 is widely expressed|
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Provided below are standard protocols that you may find useful for product applications.
PELP1 is a coactivator of estrogen receptor (see ESR1; MIM 133430)-mediated transcription and a corepressor of other nuclear hormone receptors and sequence-specific transcription factors (Choi et al., 2004 [PubMed 15456770]).
Nair, S.S., et al. EMBO Rep. 11(6):438-444(2010)
Habashy, H.O., et al. Breast Cancer Res. Treat. 120(3):603-612(2010)
Vadlamudi, R.K., et al. J. Steroid Biochem. Mol. Biol. 118 (4-5), 211-218 (2010) :
Tzelepi, V., et al. Virchows Arch. 454(1):41-53(2009)
Grivas, P.D., et al. Cell. Oncol. 31(3):235-247(2009)
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