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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   IRF9 Antibody (N-term)   

IRF9 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - IRF9 Antibody (N-term) AP11875A
    Anti-IRF9 Antibody (N-term) at 1:2000 dilution + NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - IRF9 Antibody (N-term) AP11875A
    IRF9 Antibody (N-term) (Cat. #AP11875a) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the IRF9 antibody detected the IRF9 protein (arrow).
  • IHC-P - IRF9 Antibody (N-term) AP11875A
    IRF9 Antibody (N-term) (Cat. #AP11875a)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IRF9 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • IF - IRF9 Antibody (N-term) AP11875A
    Confocal immunofluorescent analysis of IRF9 Antibody (N-term)(Cat#AP11875a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession Q00978
Other Accession NP_006075
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 43696 Da
Antigen Region 75-104 aa
Additional Information
Gene ID 10379
Other Names Interferon regulatory factor 9, IRF-9, IFN-alpha-responsive transcription factor subunit, ISGF3 p48 subunit, Interferon-stimulated gene factor 3 gamma, ISGF-3 gamma, Transcriptional regulator ISGF3 subunit gamma, IRF9, ISGF3G
Target/Specificity This IRF9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 75-104 amino acids from the N-terminal region of human IRF9.
Dilution WB~~1:1000
IHC-P~~1:10~50
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIRF9 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IRF9
Synonyms ISGF3G
Function Transcription factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. IRF9/ISGF3G associates with the phosphorylated STAT1:STAT2 dimer to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.
Cellular Location Cytoplasm. Nucleus. Note=Translocated into the nucleus upon activation by IFN-alpha/beta
Research Areas
Citations (0)

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Background

IRF9 is a transcription regulatory factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.

References

Maiwald, T., et al. FEBS J. 277(22):4741-4754(2010)
Schuurhof, A., et al. Pediatr. Pulmonol. 45(6):608-613(2010)
Watanabe, T., et al. J. Clin. Invest. 120(5):1645-1662(2010)
Mosbruger, T.L., et al. J. Infect. Dis. 201(9):1371-1380(2010)
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :

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$ 295.00
$ 99.00
Cat# AP11875A
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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