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TBC1D13 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - TBC1D13 Antibody (Center) AP11876c
    All lanes : Anti-TBC1D13 Antibody (Center) at 1:500 dilution Lane 1:PC-12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size : 47kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IF - TBC1D13 Antibody (Center) AP11876c
    Confocal immunofluorescent analysis of TBC1D13 Antibody (Center)(Cat#AP11876c) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
    detail
  • WB - TBC1D13 Antibody (Center) AP11876c
    TBC1D13 Antibody (Center) (Cat. #AP11876c) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the TBC1D13 antibody detected the TBC1D13 protein (arrow).
    detail
  • WB - TBC1D13 Antibody (Center) AP11876c
    TBC1D13 Antibody (Center) (Cat. #AP11876c) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the TBC1D13 antibody detected the TBC1D13 protein (arrow).
    detail
  • IHC-P - TBC1D13 Antibody (Center) AP11876c
    TBC1D13 Antibody (Center) (Cat. #AP11876c)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TBC1D13 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, IHC-P, E
Primary Accession Q9NVG8
Other Accession NP_060671
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 46554 Da
Antigen Region 76-105 aa
Additional Information
Gene ID 54662
Other Names TBC1 domain family member 13, TBC1D13
Target/Specificity This TBC1D13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-105 amino acids from the Central region of human TBC1D13.
Dilution WB~~1:1000
IF~~1:10~50
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTBC1D13 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TBC1D13
Function Acts as a GTPase-activating protein for RAB35. Together with RAB35 may be involved in regulation of insulin-induced glucose transporter SLC2A4/GLUT4 translocation to the plasma membrane in adipocytes.
Cellular Location Membrane {ECO:0000250|UniProtKB:Q8R3D1}. Cytoplasm {ECO:0000250|UniProtKB:Q8R3D1}
Research Areas
Citations (0)
citation

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Background

TBC1D13 may act as a GTPase-activating protein for Rab family protein(s).

References

Ishibashi, K., et al. Genes Cells 14(1):41-52(2009)

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$ 365.00
$ 140.00
Cat# AP11876c
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