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>   home   >   Products   >   Primary Antibodies   >   JHDM2a Antibody (N-term)   

JHDM2a Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - JHDM2a Antibody (N-term) AP1193a
    JHDM2a Antibody (N-term) (Cat.#AP1193a) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the JHDM2a antibody detected the JHDM2a protein (arrow).
  • IHC-P - JHDM2a Antibody (N-term) AP1193a
    JHDM2a Antibody (N-term) (Cat. #AP1193a)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of JHDM2a Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q9Y4C1
Other Accession NP_060903
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 147341 Da
Antigen Region 50-79 aa
Additional Information
Gene ID 55818
Other Names Lysine-specific demethylase 3A, 11411-, JmjC domain-containing histone demethylation protein 2A, Jumonji domain-containing protein 1A, KDM3A, JHDM2A, JMJD1, JMJD1A, KIAA0742, TSGA
Target/Specificity This JHDM2a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 50-79 amino acids from the N-terminal region of human JHDM2a.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsJHDM2a Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KDM3A
Synonyms JHDM2A, JMJD1, JMJD1A, KIAA0742, TSGA
Function Histone demethylase that specifically demethylates 'Lys- 9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.
Cellular Location Cytoplasm. Nucleus. Note=Nuclear in round spermatids. When spermatids start to elongate, localizes to the cytoplasm where it forms distinct foci which disappear in mature spermatozoa (By similarity).
Citations (0)

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Background

JHDM2a is a zinc finger protein that contains a jumonji domain. It is a histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. This protein preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. It is involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation.

References

Wellmann,S., Biochem. Biophys. Res. Commun. 372 (4), 892-897 (2008)
Yamane,K., Cell 125 (3), 483-495 (2006)

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Cat# AP1193a
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