|Application ||WB, E|
|Other Accession||A6NJW9, NP_004922|
|Calculated MW||23722 Da|
|Antigen Region||141-167 aa|
|Other Names||T-cell surface glycoprotein CD8 beta chain, CD8b, CD8B, CD8B1|
|Target/Specificity||This CD8B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 141-167 amino acids from the C-terminal region of human CD8B1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CD8B1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing.|
|Cellular Location||Isoform 1: Cell membrane; Single-pass type I membrane protein Isoform 3: Secreted. Isoform 5: Cell membrane; Single-pass type I membrane protein Isoform 7: Secreted.|
|Tissue Location||Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells.|
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Provided below are standard protocols that you may find useful for product applications.
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified.
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Chen, M.L., et al. Eur. J. Immunol. 39(12):3423-3435(2009)
Thakral, D., et al. J. Immunol. 180(11):7431-7442(2008)
Stove, V., et al. J. Virol. 79(17):11422-11433(2005)
Moody, A.M., et al. J. Biol. Chem. 278(9):7240-7246(2003)
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