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POSTN Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • FC - POSTN Antibody (C-term) AP11962b
    Overlay histogram showing Hela cells stained with AP11962B(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP11962B, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • WB - POSTN Antibody (C-term) AP11962b
    All lanes : Anti- POSTN Antibody (C-term) at 1:1000 dilution Lane 1: human breast lysate Lane 2: rat lung lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 93 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - POSTN Antibody (C-term) AP11962b
    All lanes : Anti- POSTN Antibody (C-term) at 1:1000 dilution Lane 1: human breast lysate Lane 2: rat lung lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 93 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - POSTN Antibody (C-term) AP11962b
    POSTN Antibody (C-term) (Cat. #AP11962b)immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of POSTN Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • IF - POSTN Antibody (C-term) AP11962b
    Confocal immunofluorescent analysis of POSTN Antibody (C-term)(Cat#AP11962b) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, WB, IHC-P, IF, E
Primary Accession Q15063
Other Accession NP_006466
Reactivity Human, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 93314 Da
Antigen Region 687-716 aa
Additional Information
Gene ID 10631
Other Names Periostin, PN, Osteoblast-specific factor 2, OSF-2, POSTN, OSF2
Target/Specificity This POSTN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 687-716 amino acids from the C-terminal region of human POSTN.
Dilution FC~~1:25
WB~~1:1000
IHC-P~~1:10~50
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPOSTN Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name POSTN
Synonyms OSF2
Function Induces cell attachment and spreading and plays a role in cell adhesion (PubMed:12235007). Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX (By similarity).
Cellular Location Golgi apparatus {ECO:0000250|UniProtKB:Q62009}. Secreted Secreted, extracellular space, extracellular matrix Note=Colocalizes with BMP1 in the Golgi {ECO:0000250|UniProtKB:Q62009}
Tissue Location Widely expressed with highest levels in aorta, stomach, lower gastrointestinal tract, placenta, uterus, thyroid tissue and breast. Up-regulated in epithelial ovarian tumors. Not expressed in normal ovaries. Also highly expressed at the tumor periphery of lung carcinoma tissue but not within the tumor Overexpressed in breast cancers.
Research Areas
Citations ( 0 )

Background

POSTN binds to heparin. It induces cell attachment and spreading and plays a role in cell adhesion and may play a role in extracellular matrix mineralization.

References

Zhu, M., et al. Gynecol. Oncol. 119(2):337-344(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Takada, M., et al. Biochem. Biophys. Res. Commun. 399(2):221-226(2010)
Sidhu, S.S., et al. Proc. Natl. Acad. Sci. U.S.A. 107(32):14170-14175(2010)
Tischler, V., et al. BMC Cancer 10, 273 (2010) :

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$ 295.00
$ 99.00
Cat# AP11962b
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(40 western blots)
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