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>   home   >   Products   >   Primary Antibodies   >   Developmental Biology   >   WT1 Antibody (Center E361)   

WT1 Antibody (Center E361)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • FC - WT1 Antibody (Center E361) AP11964c
    Overlay histogram showing Hela cells stained with AP11964c (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP11964c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • WB - WT1 Antibody (Center E361) AP11964c
    All lanes : Anti-WT1 Antibody (Center E361) at 1:2000 dilution Lane 1: K562 whole cell lysate Lane 2: MOLT-4 whole cell lysate Lane 3: HT-1080 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 49 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - WT1 Antibody (Center E361) AP11964c
    WT1 Antibody (Center E361) (Cat. #AP11964c) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the WT1 antibody detected the WT1 protein (arrow).
  • IHC-P - WT1 Antibody (Center E361) AP11964c
    WT1 Antibody (Center E361) (Cat. #AP11964c)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of WT1 Antibody (Center E361) for immunohistochemistry. Clinical relevance has not been evaluated.
  • IF - WT1 Antibody (Center E361) AP11964c
    Confocal immunofluorescent analysis of WT1 Antibody (Center E361)(Cat. #AP11964c) with MCF-7 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
  • FC - WT1 Antibody (Center E361) AP11964c
    WT1 Antibody (Center E361) (Cat. #AP11964c) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P19544
Other Accession P49952, O62651, P22561, P79958, B7ZSG3, NP_000369
Reactivity Human
Predicted Mouse, Rat, Pig, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 49188 Da
Antigen Region 346-375 aa
Additional Information
Gene ID 7490
Other Names Wilms tumor protein, WT33, WT1
Target/Specificity This WT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 346-375 amino acids from the Central region of human WT1.
Dilution FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsWT1 Antibody (Center E361) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name WT1
Function Transcription factor that plays an important role in cellular development and cell survival. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA.
Cellular Location Nucleus. Nucleus, nucleolus. Cytoplasm. Note=Shuttles between nucleus and cytoplasm Isoform 4: Nucleus, nucleoplasm.
Tissue Location Expressed in the kidney and a subset of hematopoietic cells
Research Areas
Citations (0)

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This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation site upstream of and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq].


Sitaram, R.T., et al. Br. J. Cancer 103(8):1255-1262(2010)
Dohi, S., et al. Anticancer Res. 30(8):3187-3192(2010)
Rocquain, J., et al. BMC Cancer 10, 401 (2010) :
Wagner, K.D., et al. J. Cell. Sci. 116 (PT 9), 1653-1658 (2003) :
Mitsuya, K., et al. Hum. Mol. Genet. 6(13):2243-2246(1997)

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Cat# AP11964c
(40 western blots)
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