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RAD54 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - RAD54 Antibody (C-term) AP11971b
    RAD54 Antibody (C-term) (Cat. #AP11971b) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the RAD54 antibody detected the RAD54 protein (arrow).
  • FC - RAD54 Antibody (C-term) AP11971b
    RAD54 Antibody (C-term) (Cat. #AP11971b) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q92698
Other Accession P70270, O12944, NP_003570
Reactivity Human
Predicted Chicken, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 84352 Da
Antigen Region 634-663 aa
Additional Information
Gene ID 8438
Other Names DNA repair and recombination protein RAD54-like, 364-, RAD54 homolog, hHR54, hRAD54, RAD54L, RAD54A
Target/Specificity This RAD54 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 634-663 amino acids from the C-terminal region of human RAD54.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRAD54 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RAD54L
Synonyms RAD54A
Function Involved in DNA repair and mitotic recombination. Functions in the recombinational DNA repair (RAD52) pathway. Dissociates RAD51 from nucleoprotein filaments formed on dsDNA. Could be involved in the turnover of RAD51 protein-dsDNA filaments (By similarity). May play also an essential role in telomere length maintenance and telomere capping in mammalian cells.
Cellular Location Nucleus.
Citations (0)

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The protein encoded by this gene belongs to the DEAD-like helicase superfamily, and shares similarity with Saccharomyces cerevisiae Rad54, a protein known to be involved in the homologous recombination and repair of DNA. This protein has been shown to play a role in homologous recombination related repair of DNA double-strand breaks. The binding of this protein to double-strand DNA induces a DNA topological change, which is thought to facilitate homologous DNA paring, and stimulate DNA recombination. Alternative splicing results in multiple transcript variants encoding the same protein.


Liu, Y., et al. Carcinogenesis 31(10):1762-1769(2010)
Briggs, F.B., et al. Am. J. Epidemiol. 172(2):217-224(2010)
Monsees, G.M., et al. Breast Cancer Res. Treat. (2010) In press :
Guey, L.T., et al. Eur. Urol. 57(2):283-292(2010)
Hosgood, H.D. III, et al. Occup Environ Med 66(12):848-853(2009)

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$ 295.00
$ 99.00
Cat# AP11971b
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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