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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   TRIM72 Antibody (C-term)   

TRIM72 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - TRIM72  Antibody (C-term) AP11980b
    All lanes : Anti-TRIM72 Antibody (C-term) at 1:2000 dilution Lane 1: HCT116 whole cell lysate Lane 2: human skeletal muscle lysate Lane 3: mouse skeletal muscle lysate Lane 4: rat skeletal muscle lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 53 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - TRIM72  Antibody (C-term) AP11980b
    TRIM72 Antibody (C-term) (Cat. #AP11980b)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TRIM72 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q6ZMU5
Other Accession NP_001008275.2
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 52731 Da
Antigen Region 299-327 aa
Additional Information
Gene ID 493829
Other Names Tripartite motif-containing protein 72, Mitsugumin-53, Mg53, TRIM72 (HGNC:32671), MG53
Target/Specificity This TRIM72 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 299-327 amino acids from the C-terminal region of human TRIM72.
Dilution WB~~1:2000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTRIM72 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TRIM72 (HGNC:32671)
Synonyms MG53
Function Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca(2+)-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles (By similarity).
Cellular Location Cell membrane, sarcolemma. Cytoplasmic vesicle membrane. Note=Tethered to plasma membrane and cytoplasmic vesicles via its interaction with phosphatidylserine.
Research Areas
Citations (0)

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Background

TRIM72 is a muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca(2+)-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles (By similarity).

References

Park, E.Y., et al. Proteins 78(3):790-795(2010)
Han, S., et al. Hum. Mol. Genet. 18(6):1171-1180(2009)
Martin, J., et al. Nature 432(7020):988-994(2004)

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$ 295.00
$ 99.00
Cat# AP11980b
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(40 western blots)
Availability: In Stock
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