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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   B3GAT3 Antibody (C-term)   

B3GAT3 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - B3GAT3 Antibody (C-term) AP12032b
    B3GAT3 Antibody (C-term) (Cat. #AP12032b) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the B3GAT3 antibody detected the B3GAT3 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O94766
Other Accession P58158, Q9WU47, NP_036332.2
Reactivity Human
Predicted Hamster, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 37122 Da
Antigen Region 293-321 aa
Additional Information
Gene ID 26229
Other Names Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3, Beta-1, 3-glucuronyltransferase 3, Glucuronosyltransferase I, GlcAT-I, UDP-GlcUA:Gal beta-1, 3-Gal-R glucuronyltransferase, GlcUAT-I, B3GAT3
Target/Specificity This B3GAT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 293-321 amino acids from the C-terminal region of human B3GAT3.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsB3GAT3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name B3GAT3
Function Glycosaminoglycans biosynthesis. Involved in forming the linkage tetrasaccharide present in heparan sulfate and chondroitin sulfate. Transfers a glucuronic acid moiety from the uridine diphosphate-glucuronic acid (UDP-GlcUA) to the common linkage region trisaccharide Gal-beta-1,3-Gal-beta-1,4-Xyl covalently bound to a Ser residue at the glycosaminylglycan attachment site of proteoglycans. Can also play a role in the biosynthesis of l2/HNK-1 carbohydrate epitope on glycoproteins. Shows strict specificity for Gal-beta-1,3-Gal-beta-1,4-Xyl, exhibiting negligible incorporation into other galactoside substrates including Galbeta1-3Gal beta1-O-benzyl, Galbeta1-4GlcNAc and Galbeta1-4Glc. Stimulates 2-phosphoxylose phosphatase activity of PXYLP1 in presence of uridine diphosphate-glucuronic acid (UDP- GlcUA) during completion of linkage region formation.
Cellular Location Golgi apparatus membrane; Single-pass type II membrane protein. Golgi apparatus, cis-Golgi network
Tissue Location Ubiquitous (but weakly expressed in all tissues examined)
Research Areas
Citations (0)

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The protein encoded by this gene is a member of the glucuronyltransferase gene family, enzymes that exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product catalyzes the formation of the glycosaminoglycan-protein linkage by way of a glucuronyl transfer reaction in the final step of the biosynthesis of the linkage region of proteoglycans.


Tone, Y., et al. J. Biol. Chem. 283(24):16801-16807(2008)
Fondeur-Gelinotte, M., et al. Glycobiology 17(8):857-867(2007)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Gulberti, S., et al. J. Biol. Chem. 280(2):1417-1425(2005)
Venkatesan, N., et al. Proc. Natl. Acad. Sci. U.S.A. 101(52):18087-18092(2004)

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Cat# AP12032b
(40 western blots)
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