|Application ||WB, E|
|Other Accession||P58158, Q9WU47, NP_036332.2|
|Calculated MW||37122 Da|
|Antigen Region||293-321 aa|
|Other Names||Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3, Beta-1, 3-glucuronyltransferase 3, Glucuronosyltransferase I, GlcAT-I, UDP-GlcUA:Gal beta-1, 3-Gal-R glucuronyltransferase, GlcUAT-I, B3GAT3|
|Target/Specificity||This B3GAT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 293-321 amino acids from the C-terminal region of human B3GAT3.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||B3GAT3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Glycosaminoglycans biosynthesis. Involved in forming the linkage tetrasaccharide present in heparan sulfate and chondroitin sulfate. Transfers a glucuronic acid moiety from the uridine diphosphate-glucuronic acid (UDP-GlcUA) to the common linkage region trisaccharide Gal-beta-1,3-Gal-beta-1,4-Xyl covalently bound to a Ser residue at the glycosaminylglycan attachment site of proteoglycans. Can also play a role in the biosynthesis of l2/HNK-1 carbohydrate epitope on glycoproteins. Shows strict specificity for Gal-beta-1,3-Gal-beta-1,4-Xyl, exhibiting negligible incorporation into other galactoside substrates including Galbeta1-3Gal beta1-O-benzyl, Galbeta1-4GlcNAc and Galbeta1-4Glc. Stimulates 2-phosphoxylose phosphatase activity of PXYLP1 in presence of uridine diphosphate-glucuronic acid (UDP- GlcUA) during completion of linkage region formation.|
|Cellular Location||Golgi apparatus membrane; Single-pass type II membrane protein. Golgi apparatus, cis-Golgi network|
|Tissue Location||Ubiquitous (but weakly expressed in all tissues examined)|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is a member of the glucuronyltransferase gene family, enzymes that exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product catalyzes the formation of the glycosaminoglycan-protein linkage by way of a glucuronyl transfer reaction in the final step of the biosynthesis of the linkage region of proteoglycans.
Tone, Y., et al. J. Biol. Chem. 283(24):16801-16807(2008)
Fondeur-Gelinotte, M., et al. Glycobiology 17(8):857-867(2007)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Gulberti, S., et al. J. Biol. Chem. 280(2):1417-1425(2005)
Venkatesan, N., et al. Proc. Natl. Acad. Sci. U.S.A. 101(52):18087-18092(2004)
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.