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SAMD8 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - SAMD8 Antibody (N-term) AP12035a
    Anti-SAMD8 Antibody (N-term) at 1:1000 dilution + human heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - SAMD8 Antibody (N-term) AP12035a
    Western blot analysis of SAMD8 Antibody (N-term) Pab (Cat. #AP12035a) pre-incubated without(lane 1) and with(lane 2) blocking peptide in mouse liver tissue lysate. SAMD8 Antibody (N-term) (arrow) was detected using the purified Pab.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q96LT4
Other Accession Q9DA37, NP_653261.1
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 48321 Da
Antigen Region 38-67 aa
Additional Information
Gene ID 142891
Other Names Sphingomyelin synthase-related protein 1, SMSr, 278-, Ceramide phosphoethanolamine synthase, CPE synthase, Sterile alpha motif domain-containing protein 8, SAM domain-containing protein 8, SAMD8
Target/Specificity This SAMD8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 38-67 amino acids from the N-terminal region of human SAMD8.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSAMD8 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SAMD8
Function Sphingomyelin synthases synthesize sphingolipids through transfer of a phosphatidyl head group on to the primary hydroxyl of ceramide. SAMD8 is an endoplasmic reticulum (ER) transferase that has no sphingomyelin synthase activity but can convert phosphatidylethanolamine (PE) and ceramide to ceramide phosphoethanolamine (CPE) albeit with low product yield. Appears to operate as a ceramide sensor to control ceramide homeostasis in the endoplasmic reticulum rather than a converter of ceramides. Seems to be critical for the integrity of the early secretory pathway.
Cellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein
Research Areas
Citations (0)

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Grupe, A., et al. Am. J. Hum. Genet. 78(1):78-88(2006)
Barrios-Rodiles, M., et al. Science 307(5715):1621-1625(2005)
Huitema, K., et al. EMBO J. 23(1):33-44(2004)

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$ 295.00
$ 99.00
Cat# AP12035a
(40 western blots)
Availability: In Stock
Bulk Size
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