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AP1M1 Antibody (Center)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP12036c | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP12036c-ev20 | Ev20 size 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
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- Backgrounds
AP1M1 Antibody (Center) - Product info | |
| Application | WB, IHC, IF
|
| Primary Accession | Q9BXS5 |
| Other Accession | Q32Q06, P35585, Q2KJ81, NP_115882.1 |
| Reactivity | Human |
| Predicted | Bovine, Mouse, Rat |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 48587 Da |
AP1M1 Antibody (Center) - Additional info | |
| Gene ID 8907 | |
| Other Names AP1M1; CLTNM; AP-1 complex subunit mu-1; AP-mu chain family member mu1A; Adaptor protein complex AP-1 mu-1 subunit; Adaptor-related protein complex 1 mu-1 subunit; Clathrin assembly protein complex 1 medium chain 1; Clathrin coat assembly protein AP47; Clathrin coat-associated protein AP47; Golgi adaptor HA1/AP1 adaptin mu-1 subunit; Mu-adaptin 1; Mu1A-adaptin | |
| Target/Specificity This AP1M1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 206-235 amino acids from the Central region of human AP1M1. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:10~50 IF~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions AP1M1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. | |
AP1M1 Antibody (Center) - Protein Information | |
| Name AP1M1 | |
| Synonyms CLTNM | |
| Function Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules | |
| Cellular Location Golgi apparatus. Cytoplasmic vesicle, clathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Note=Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex | |
AP1M1 Antibody (Center) - Related products
AP1M1 Antibody (Center) - Application data
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AP1M1 Antibody (Center) (Cat. #AP12036c) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the AP1M1 antibody detected the AP1M1 protein (arrow).
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AP1M1 Antibody (Center) (Cat. #AP12036c)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of AP1M1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
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Confocal immunofluorescent analysis of AP1M1 Antibody (Center)(Cat#AP12036c) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
AP1M1 Antibody (Center) - Research Areas
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BACKGROUND
The protein encoded by this gene is the medium chain of the trans-Golgi network clathrin-associated protein complex AP-1. The other components of this complex are beta-prime-adaptin, gamma-adaptin, and the small chain AP1S1. This complex is located at the Golgi vesicle and links clathrin to receptors in coated vesicles. These vesicles are involved in endocytosis and Golgi processing. Alternatively spliced transcript variants encoding distinct protein isoforms have been found for this gene. [provided by RefSeq].
REFERENCES
Sawasdee, N., et al. Biochem. Biophys. Res. Commun. 401(1):85-91(2010) Venkatesan, K., et al. Nat. Methods 6(1):83-90(2009) Noviello, C.M., et al. J. Virol. 82(3):1249-1258(2008) Medigeshi, G.R., et al. Traffic 9(1):121-132(2008) Roeth, J.F., et al. J. Cell Biol. 167(5):903-913(2004)
