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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   KLF4 Antibody (Center)   

KLF4 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - KLF4 Antibody (Center) AP12069c
    KLF4 Antibody (Center) (Cat. #AP12069c) western blot analysis in MCF-7,K562 cell line lysates (35ug/lane).This demonstrates the KLF4 antibody detected the KLF4 protein (arrow).
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession O43474
Other Accession NP_004226.3
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 54671 Da
Antigen Region 283-314 aa
Additional Information
Gene ID 9314
Other Names Krueppel-like factor 4, Epithelial zinc finger protein EZF, Gut-enriched krueppel-like factor, KLF4, EZF, GKLF
Target/Specificity This KLF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 283-314 amino acids from the Central region of human KLF4.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsKLF4 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KLF4
Synonyms EZF, GKLF
Function Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.
Cellular Location Nucleus.
Research Areas
Citations (0)

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Background

KLF4 is a transcription factor which acts as both an activator and repressor. Binds the CACCC core sequence. Binds to multiple sites in the 5'-flanking region of its own gene and can activate its own transcription. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development.

References

Guan, H., et al. Blood 116(9):1469-1478(2010)
Wong, C.W., et al. Stem Cells 28(9):1510-1517(2010)
Ohnesorge, N., et al. J. Biol. Chem. 285(34):26199-26210(2010)
Cekanova, M., et al. Eur. J. Pharmacol. 637 (1-3), 30-37 (2010) :
Yori, J.L., et al. J. Biol. Chem. 285(22):16854-16863(2010)

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Cat# AP12069c
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(40 western blots)
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