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SPECC1 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - SPECC1 Antibody (Center) AP12147c
    SPECC1 Antibody (Center) (Cat. #AP12147c) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the SPECC1 antibody detected the SPECC1 protein (arrow).
  • WB - SPECC1 Antibody (Center) AP12147c
    SPECC1 Antibody (Center) (Cat. #AP12147c) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the SPECC1 antibody detected the SPECC1 protein (arrow).
  • IHC-P - SPECC1 Antibody (Center) AP12147c
    SPECC1 Antibody (Center) (Cat. #AP12147c)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SPECC1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - SPECC1 Antibody (Center) AP12147c
    SPECC1 Antibody (Center) (Cat. #AP12147c) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • IF - SPECC1 Antibody (Center) AP12147c
    Confocal immunofluorescent analysis of SPECC1 Antibody (Center)(Cat#AP12147c) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q69YQ0
Other Accession Q2KN98, Q2KN93, Q2KN97, NP_001138940.1
Reactivity Human, Mouse
Predicted Chicken, Zebrafish
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 124602 Da
Antigen Region 705-733 aa
Additional Information
Gene ID 23384
Other Names Cytospin-A, Renal carcinoma antigen NY-REN-22, Sperm antigen with calponin homology and coiled-coil domains 1-like, SPECC1-like protein, SPECC1L, CYTSA, KIAA0376
Target/Specificity This SPECC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 705-733 amino acids from the Central region of human SPECC1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSPECC1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms CYTSA, KIAA0376
Function Involved in cytokinesis and spindle organization. May play a role in actin cytoskeleton organization and microtubule stabilization and hence required for proper cell adhesion and migration.
Cellular Location Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, spindle. Cell junction, gap junction. Note=Colocalizes with acetylated alpha-tubulin, gamma-tubulin and F-actin. Also observed in a ring around gamma-tubulin containing centrioles possibly in the microtubule organizing center
Research Areas
Citations (0)

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CYTSAis involved in cytokinesis and spindle organization.


Scanlan, M.J., et al. Int. J. Cancer 83(4):456-464(1999)

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Cat# AP12147c
(40 western blots)
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