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NEU2 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - NEU2 Antibody (N-term) AP12189A
    Anti-NEU2 Antibody (N-term) at 1:1000 dilution + human fetal liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - NEU2 Antibody (N-term) AP12189A
    NEU2 Antibody (N-term) (Cat. #AP12189a) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the NEU2 antibody detected the NEU2 protein (arrow).
  • IHC-P - NEU2 Antibody (N-term) AP12189A
    NEU2 Antibody (N-term) (Cat. #AP12189a)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NEU2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • IF - NEU2 Antibody (N-term) AP12189A
    Confocal immunofluorescent analysis of NEU2 Antibody (N-term)(Cat#AP12189a) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
  • FC - NEU2 Antibody (N-term) AP12189A
    NEU2 Antibody (N-term) (Cat. #AP12189a) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9Y3R4
Other Accession NP_005374.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 42254 Da
Antigen Region 23-50 aa
Additional Information
Gene ID 4759
Other Names Sialidase-2, Cytosolic sialidase, N-acetyl-alpha-neuraminidase 2, NEU2
Target/Specificity This NEU2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-50 amino acids from the N-terminal region of human NEU2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNEU2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NEU2
Function Catalyzes the removal of sialic acid (N-acetylneuraminic acid) moities from glycoproteins, oligosaccharides and gangliosides.
Cellular Location Cytoplasm.
Tissue Location Expressed in skeletal muscle, fetal liver and embryonic carcinoma cell line NT2-D1
Research Areas
Citations (0)

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This gene belongs to a family of glycohydrolytic enzymes which remove sialic acid residues from glycoproteins and glycolipids. Expression studies in COS7 cells confirmed that this gene encodes a functional sialidase. Its cytosolic localization was demonstrated by cell fractionation experiments. [provided by RefSeq].


Stoppani, E., et al. Cell Biol. Int. 33(9):1020-1025(2009)
Li, C.Y., et al. Cell Res. 17(4):357-362(2007)
Chavas, L.M., et al. J. Biol. Chem. 280(1):469-475(2005)
Seyrantepe, V., et al. J. Biol. Chem. 279(35):37021-37029(2004)
Tringali, C., et al. J. Biol. Chem. 279(5):3169-3179(2004)

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$ 295.00
$ 99.00
Cat# AP12189A
(40 western blots)
Availability: In Stock
Bulk Size
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