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MED14 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - MED14 Antibody (Center) AP12202c
    MED14 Antibody (Center) (Cat. #AP12202c) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the MED14 antibody detected the MED14 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O60244
Other Accession NP_004220
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 160607 Da
Antigen Region 586-615 aa
Additional Information
Gene ID 9282
Other Names Mediator of RNA polymerase II transcription subunit 14, Activator-recruited cofactor 150 kDa component, ARC150, Cofactor required for Sp1 transcriptional activation subunit 2, CRSP complex subunit 2, Mediator complex subunit 14, RGR1 homolog, hRGR1, Thyroid hormone receptor-associated protein complex 170 kDa component, Trap170, Transcriptional coactivator CRSP150, Vitamin D3 receptor-interacting protein complex 150 kDa component, DRIP150, MED14
Target/Specificity This MED14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 586-615 amino acids from the Central region of human MED14.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMED14 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MED14
Function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Cellular Location Nucleus.
Tissue Location Ubiquitous.
Citations (0)

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The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein contains a bipartite nuclear localization signal. This gene is known to escape chromosome X-inactivation.


Wu, C., et al. Proteomics 7(11):1775-1785(2007)
Lee, J., et al. Arch. Biochem. Biophys. 461(2):200-210(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Chen, W., et al. Mol. Endocrinol. 20(3):560-572(2006)

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$ 295.00
$ 99.00
Cat# AP12202c
(40 western blots)
Availability: In Stock
Bulk Size
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