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SMPD1 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • FC - SMPD1 Antibody (C-term) AP12227b
    Overlay histogram showing K562 cells stained with AP12227b (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP12227b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti--Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • WB - SMPD1 Antibody (C-term) AP12227b
    All lanes : Anti-SMPD1 Antibody (S376) at 1:2000 dilution Lane 1: human cerebellum lysate Lane 2: HepG2 whole cell lysate Lane 3: K562 whole cell lysate Lane 4: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 70 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - SMPD1 Antibody (C-term) AP12227b
    SMPD1 Antibody (C-term) (Cat. #AP12227b)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SMPD1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P17405
Other Accession Q0VD19, NP_000534.3
Reactivity Human
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 69752 Da
Antigen Region 391-419 aa
Additional Information
Gene ID 6609
Other Names Sphingomyelin phosphodiesterase, Acid sphingomyelinase, aSMase, SMPD1, ASM
Target/Specificity This SMPD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 391-419 amino acids from the C-terminal region of human SMPD1.
Dilution FC~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSMPD1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SMPD1
Synonyms ASM
Function Converts sphingomyelin to ceramide. Also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol. Isoform 2 and isoform 3 have lost catalytic activity.
Cellular Location Lysosome.
Research Areas
Citations ( 0 )


The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified. [provided by RefSeq].


Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010) Desnick, J.P., et al. Mol. Med. 16 (7-8), 316-321 (2010) : Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009) Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007) Sleat, D.E., et al. Mol. Cell Proteomics 5(4):686-701(2006)

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$ 295.00
$ 99.00
Cat# AP12227b
(40 western blots)
Availability: In Stock
Bulk Size
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