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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   ARHB Antibody (Center)   

ARHB Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ARHB Antibody (Center) AP12264c
    Anti-ARHB Antibody (Center) at 1:4000 dilution + human brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - ARHB Antibody (Center) AP12264c
    ARHB Antibody (Center) (Cat. #AP12264c)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ARHB Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P62745
Other Accession P62747, P62746, Q3ZBW5, NP_004031.1
Reactivity Human
Predicted Bovine, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 22123 Da
Antigen Region 104-137 aa
Additional Information
Gene ID 388
Other Names Rho-related GTP-binding protein RhoB, Rho cDNA clone 6, h6, RHOB, ARH6, ARHB
Target/Specificity This ARHB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-137 amino acids from the Central region of human ARHB.
Dilution WB~~1:4000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsARHB Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RHOB
Synonyms ARH6, ARHB
Function Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development. Serves as a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Required for genotoxic stress-induced cell death in breast cancer cells.
Cellular Location Late endosome membrane; Lipid-anchor. Cell membrane; Lipid-anchor. Nucleus. Cleavage furrow. Note=Late endosomal membrane (geranylgeranylated form). Plasma membrane (farnesylated form). Also detected at the nuclear margin and in the nucleus. Translocates to the equatorial region before furrow formation in a ECT2-dependent manner
Research Areas
Citations (0)

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Background

ARHB mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development.

References

Adly, M.A., et al. J. Cutan. Pathol. 37(7):751-757(2010)
Connolly, E.C., et al. Mol. Cancer Res. 8(5):691-700(2010)
Zintzaras, E., et al. Am. J. Epidemiol. 171(8):851-858(2010)
Kim, C.H., et al. Biochem. Biophys. Res. Commun. 391(2):1182-1186(2010)
Takefuji, M., et al. J. Hum. Genet. 55(1):42-49(2010)

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$ 295.00
$ 99.00
Cat# AP12264c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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