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>   home   >   Products   >   Primary Antibodies   >   CYP27B1 Antibody (C-term)   

CYP27B1 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CYP27B1 Antibody (C-term) AP12284B
    CYP27B1 Antibody (C-term) (Cat. #AP12284b) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CYP27B1 antibody detected the CYP27B1 protein (arrow).
  • IHC-P - CYP27B1 Antibody (C-term) AP12284B
    CYP27B1 Antibody (C-term) (Cat. #AP12284b)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CYP27B1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession O15528
Other Accession NP_000776.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 56504 Da
Antigen Region 400-429 aa
Additional Information
Gene ID 1594
Other Names 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial, 25-OHD-1 alpha-hydroxylase, 25-hydroxyvitamin D(3) 1-alpha-hydroxylase, VD3 1A hydroxylase, Calcidiol 1-monooxygenase, Cytochrome P450 subfamily XXVIIB polypeptide 1, Cytochrome P450C1 alpha, Cytochrome P450VD1-alpha, Cytochrome p450 27B1, CYP27B1, CYP1ALPHA, CYP27B
Target/Specificity This CYP27B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 400-429 amino acids of human CYP27B1.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCYP27B1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CYP27B1
Synonyms CYP1ALPHA, CYP27B
Function Catalyzes the conversion of 25-hydroxyvitamin D3 (25(OH)D3) to 1-alpha,25-dihydroxyvitamin D3 (1alpha,25(OH)(2)D3), and of 24,25-dihydroxyvitamin D3 (24,25(OH)(2)D3) to 1- alpha,24,25-trihydroxyvitamin D3 (1alpha,24,25(OH)(3)D3). Is also active with 25-hydroxy-24-oxo-vitamin D3. Plays an important role in normal bone growth, calcium metabolism, and tissue differentiation.
Cellular Location Mitochondrion membrane.
Tissue Location Kidney.
Citations (0)

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Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the inner mitochondrial membrane where it hydroxylates 25-hydroxyvitamin D3 at the 1alpha position. This reaction synthesizes 1alpha,25-dihydroxyvitamin D3, the active form of vitamin D3, which binds to the vitamin D receptor and regulates calcium metabolism. Thus this enzyme regulates the level of biologically active vitamin D and plays an important role in calcium homeostasis. Mutations in this gene can result in vitamin D-dependent rickets type I.

References

Giroux, S., et al. Bone 47(5):975-981(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Holt, S.K., et al. Prostate 70(13):1448-1460(2010)
Alzahrani, A.S., et al. J. Clin. Endocrinol. Metab. 95(9):4176-4183(2010)
Sundqvist, E., et al. Eur. J. Hum. Genet. (2010) In press :

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Discontinued
Cat# AP12284B
Size:
(40 western blots)
Alternative Products:

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