|Application ||WB, IHC-P, E|
|Other Accession||Q63679, Q6PCM1, NP_001140160.1, NP_060903.2|
|Calculated MW||147341 Da|
|Antigen Region||976-1008 aa|
|Other Names||Lysine-specific demethylase 3A, 11411-, JmjC domain-containing histone demethylation protein 2A, Jumonji domain-containing protein 1A, KDM3A, JHDM2A, JMJD1, JMJD1A, KIAA0742, TSGA|
|Target/Specificity||This JHDM2a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 976-1008 amino acids from the C-terminal region of human JHDM2a.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||JHDM2a Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||JHDM2A, JMJD1, JMJD1A, KIAA0742, TSGA|
|Function||Histone demethylase that specifically demethylates 'Lys- 9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.|
|Cellular Location||Cytoplasm. Nucleus. Note=Nuclear in round spermatids. When spermatids start to elongate, localizes to the cytoplasm where it forms distinct foci which disappear in mature spermatozoa (By similarity).|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
KDM3A is a zinc finger protein that contains a jumonji domain and may play a role in hormone-dependent transcriptional activation.
Suikki, H.E., et al. Prostate 70(8):889-898(2010)
Aston, K.I., et al. Hum. Reprod. 25(6):1383-1397(2010)
Chen, H., et al. J. Biol. Chem. 285(10):7374-7383(2010)
Krieg, A.J., et al. Mol. Cell. Biol. 30(1):344-353(2010)
Sar, A., et al. Cell Tissue Res. 337(2):223-234(2009)
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