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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   SENP3 Antibody (N-term)   

SENP3 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - SENP3 Antibody (N-term) AP1234a
    Western blot analysis of SENP3 N-term polyclonal antibody (Cat. #AP1234a) in Saos-2 cell lysate. SENP3 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
  • IHC-P - SENP3 Antibody (N-term) AP1234a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • IHC-P - SENP3 Antibody (N-term) AP1234a
    Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with SENP3 antibody (N-term) (Cat.#AP1234a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q9H4L4
Other Accession Q9EP97
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 65010 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 26168
Other Names Sentrin-specific protease 3, SUMO-1-specific protease 3, Sentrin/SUMO-specific protease SENP3, SENP3, SSP3, SUSP3
Target/Specificity This SENP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human SENP3.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSENP3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SENP3
Synonyms SSP3, SUSP3
Function Protease that releases SUMO2 and SUMO3 monomers from sumoylated substrates, but has only weak activity against SUMO1 conjugates. Deconjugates SUMO2 from MEF2D, which increases its transcriptional activation capability. Deconjugates SUMO2 and SUMO3 from CDCA8. Redox sensor that, when redistributed into nucleoplasm, can act as an effector to enhance HIF1A transcriptional activity by desumoylating EP300. Required for rRNA processing through deconjugation of SUMO2 and SUMO3 from nucleophosmin, NPM1. Plays a role in the regulation of sumoylation status of ZNF148. Functions as a component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes.
Cellular Location Nucleus, nucleolus. Nucleus, nucleoplasm Cytoplasm {ECO:0000250|UniProtKB:Q9EP97}. Note=Redistributes between the nucleolus and the nucleoplasm in response to mild oxidative stress (PubMed:19680224). Mainly found in the nucleoplasm, with low levels detected in the cytoplasmic and chromatin fractions (By similarity) {ECO:0000250|UniProtKB:Q9EP97, ECO:0000269|PubMed:19680224}
Research Areas
Citations (0)

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Background

SENP3 releases SUMO2 and SUMO3 monomers from sumoylated substrates, but has only weak activity against SUMO1 conjugates. This protein deconjugates SUMO2 from MEF2D, which increases its transcriptional activation capability.

References

Muller S, et al., Nat Rev Mol Cell Biol. 2001 2(3):202-10 Review.
Hochstrasser M. Cell. 2001 107(1):5-8. Review.
Kahyo T, et al., Mol Cell. 2001 Sep;8(3):713-8.
Yeh ET, et al., Gene. 2000 May 2;248(1-2):1-14. Review.
Keane,M.M., et al., Oncogene 18 (22), 3365-3375 (1999)

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Cat# AP1234a
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