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SLC12A6 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | Q9UHW9 |
| Other Accession | NP_598408.1, NP_005126.1 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 127617 Da |
| Antigen Region | 13-42 aa |
| Gene ID | 9990 |
|---|---|
| Other Names | Solute carrier family 12 member 6, Electroneutral potassium-chloride cotransporter 3, K-Cl cotransporter 3, SLC12A6, KCC3 |
| Target/Specificity | This SLC12A6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-42 amino acids from the N-terminal region of human SLC12A6. |
| Dilution | WB~~1:1000 IHC-P~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | SLC12A6 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SLC12A6 (HGNC:10914) |
|---|---|
| Function | [Isoform 1]: Mediates electroneutral potassium-chloride cotransport when activated by cell swelling (PubMed:16048901, PubMed:11551954, PubMed:10600773, PubMed:19665974, PubMed:18566107, PubMed:21628467, PubMed:27485015). May contribute to cell volume homeostasis in single cells (PubMed:16048901, PubMed:27485015). |
| Cellular Location | Cell membrane; Multi-pass membrane protein. Basolateral cell membrane {ECO:0000250|UniProtKB:Q924N4}; Multi-pass membrane protein {ECO:0000250|UniProtKB:Q924N4} |
| Tissue Location | Expressed in brain (at protein level) (PubMed:21628467). Highly expressed in heart, brain and kidney Detected at lower levels in skeletal muscle, placenta, lung and pancreas (PubMed:10600773). Detected in umbilical vein endothelial cells (PubMed:16048901). [Isoform 5]: Testis specific. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene is a member of the K-Cl cotransporter (KCC) family. K-Cl cotransporters are integral membrane proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The proteins encoded by this gene are activated by cell swelling induced by hypotonic conditions. Alternate splicing results in multiple transcript variants encoding different isoforms. Mutations in this gene are associated with agenesis of the corpus callosum with peripheral neuropathy.
References
Rinehart, J., et al. Cell 138(3):525-536(2009)
Rudnik-Schoneborn, S., et al. Neuropediatrics 40(3):129-133(2009)
de Krom, M., et al. Biol. Psychiatry 65(7):625-630(2009)
Moser, D., et al. Neuropsychopharmacology 34(2):458-467(2009)
Salin-Cantegrel, A., et al. Hum. Mol. Genet. 17(17):2703-2711(2008)
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