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PIAS1 Antibody (N-term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
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| Catalog # | Size | Availability | Price | |
| AP1242a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
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PIAS1 Antibody (N-term) - Product info | |
| Application | WB, IHC
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| Primary Accession | O75925 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 71836 Da |
PIAS1 Antibody (N-term) - Additional info | |
| Gene ID 8554 | |
| Other Names PIAS1; DDXBP1; E3 SUMO-protein ligase PIAS1; DEAD/H box-binding protein 1; Gu-binding protein; Protein inhibitor of activated STAT protein 1; RNA helicase II-binding protein | |
| Target/Specificity This PIAS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 108~137 amino acids from the N-terminal region of human PIAS1. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:50~100 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions PIAS1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
PIAS1 Antibody (N-term) - Protein Information | |
| Name PIAS1 | |
| Synonyms DDXBP1 | |
| Function Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context Together with PRMT1, may repress STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling | |
| Cellular Location Nucleus speckle. Note=Interaction with CSRP2 may induce a partial redistribution along the cytoskeleton | |
| Tissue Location Expressed in numerous tissues with highest level in testis | |
PIAS1 Antibody (N-term) - Related products
AP1242a: PIAS1 Antibody (N-term)
AP1243a: PIAS1 Antibody (C-term)
RI13977: PIAS1 predesign siRNA
DC14241: Human PIAS1 cDNA Clone
LY12209a: PIAS1 Over-expression Lysate
BP1242a: PIAS1 Antibody (N-term) Blocking Peptide
PIAS1 Antibody (N-term) - Application data
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The PIAS1 polyclonal antibody (Cat. #AP1242a) is used in Western blot to detect PIAS1 in HL-60 tissue lysate.
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Western blot analysis of PIAS1 (arrow) using rabbit polyclonal PIAS1 Antibody (N-term) (RB00668). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIAS1 gene (Lane 2) (Origene Technologies).
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
PIAS1 Antibody (N-term) - Research Areas
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BACKGROUND
PIAS1 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. This protein plays a crucial role in transcriptional coregulation of various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in androgen receptor initiation and maintenance of spermatogenesis. The effects of transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context.
REFERENCES
Miyauchi, Y., et al., J. Biol. Chem. 277(51):50131-50136 (2002). Nishida, T., et al., J. Biol. Chem. 277(44):41311-41317 (2002). Tan, J.A., et al., J. Biol. Chem. 277(19):16993-17001 (2002). Megidish, T., et al., J. Biol. Chem. 277(10):8255-8259 (2002). Liu, B., et al., J. Biol. Chem. 276(39):36624-36631 (2001).
