- CITATIONS: 1
|Application ||WB, IHC-P, E|
|Other Accession||Q5M807, O35445, NP_008844.1|
|Calculated MW||19881 Da|
|Antigen Region||65-94 aa|
|Other Names||E3 ubiquitin-protein ligase RNF5, 632-, Protein G16, RING finger protein 5, Ram1 homolog, HsRma1, RNF5, G16, NG2, RMA1|
|Target/Specificity||This RNF5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 65-94 amino acids from the Central region of human RNF5.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RNF5 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||G16, NG2, RMA1|
|Function||Has E2-dependent E3 ubiquitin-protein ligase activity. May function together with E2 ubiquitin-conjugating enzymes UBE2D1/UBCH5A and UBE2D2/UBC4. Mediates ubiquitination of PXN/paxillin and Salmonella type III secreted protein sopA. May be involved in regulation of cell motility and localization of PXN/paxillin. Mediates the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD; the ubiquitination appears to involve E2 ubiquitin-conjugating enzyme UBE2N. Mediates the 'Lys-48'-linked polyubiquitination of TMEM173 at 'Lys-150' leading to its proteasomal degradation; the ubiquitination occurrs in mitochondria after viral transfection and regulates antiviral responses.|
|Cellular Location||Membrane; Multi-pass membrane protein. Mitochondrion membrane. Endoplasmic reticulum membrane Note=Predominantly located in the plasma membrane, with some localization occurring within cytoplasmic organelles|
|Tissue Location||Widely expressed.|
Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene contains a RING finger, which is a motif known to be involved in protein-protein interactions. This protein is a membrane-bound ubiquitin ligase. It can regulate cell motility by targeting paxillin ubiquitination and altering the distribution and localization of paxillin in cytoplasm and cell focal adhesions.
Barcellos, L.F., et al. PLoS Genet. 5 (10), E1000696 (2009) :
Tcherpakov, M., et al. J. Biol. Chem. 284(18):12099-12109(2009)
Zhong, B., et al. Immunity 30(3):397-407(2009)
McKinnon, E., et al. Diabetes Obes Metab 11 SUPPL 1, 92-100 (2009) :
Bromberg, K.D., et al. Cancer Res. 67(17):8172-8179(2007)
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