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RNF5 Antibody (Center)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP12440c | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP12440c-ev20 | 20 ug 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
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- Backgrounds
RNF5 Antibody (Center) - Product info | |
| Application | WB, IHC
|
| Primary Accession | Q99942 |
| Other Accession | Q5M807, O35445, NP_008844.1 |
| Reactivity | Human |
| Predicted | Mouse, Rat |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 19881 Da |
RNF5 Antibody (Center) - Additional info | |
| Gene ID 6048 | |
| Other Names RNF5; G16; NG2; RMA1; E3 ubiquitin-protein ligase RNF5; Protein G16; RING finger protein 5; Ram1 homolog | |
| Target/Specificity This RNF5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 64-94 amino acids from the Central region of human RNF5. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions RNF5 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. | |
RNF5 Antibody (Center) - Protein Information | |
| Name RNF5 | |
| Synonyms G16, NG2, RMA1 | |
| Function Has E2-dependent E3 ubiquitin-protein ligase activity. May function together with E2 ubiquitin-conjugating enzymes UBE2D1/UBCH5A and UBE2D2/UBC4. Mediates ubiquitination of PXN/paxillin and Salmonella type III secreted protein sopA. May be involved in regulation of cell motility and localization of PXN/paxillin. Mediates the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD; the ubiquitination appears to involve E2 ubiquitin-conjugating enzyme UBE2N. Mediates the 'Lys-48'-linked polyubiquitination of TMEM173 at 'Lys-150' leading to its proteasomal degradation; the ubiquitination occurrs in mitochondria after viral transfection and regulates antiviral responses | |
| Cellular Location Membrane; Multi-pass membrane protein. Mitochondrion membrane. Endoplasmic reticulum membrane Note=Predominantly located in the plasma membrane, with some localization occurring within cytoplasmic organelles | |
| Tissue Location Widely expressed. | |
RNF5 Antibody (Center) - Related products
AP12440c: RNF5 Antibody (Center)
AP8608a: RNF5 Antibody (N-term)
DC01399: Human RNF5 cDNA Clone
RNF5 Antibody (Center) - Application data
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Western blot analysis of RNF5 (arrow) using rabbit polyclonal RNF5 Antibody (Center) (Cat. #AP12440c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RNF5 gene.
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RNF5 Antibody (Center) (Cat. #AP12440c)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RNF5 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
RNF5 Antibody (Center) - Research Areas
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BACKGROUND
The protein encoded by this gene contains a RING finger, which is a motif known to be involved in protein-protein interactions. This protein is a membrane-bound ubiquitin ligase. It can regulate cell motility by targeting paxillin ubiquitination and altering the distribution and localization of paxillin in cytoplasm and cell focal adhesions.
REFERENCES
Barcellos, L.F., et al. PLoS Genet. 5 (10), E1000696 (2009) : Tcherpakov, M., et al. J. Biol. Chem. 284(18):12099-12109(2009) Zhong, B., et al. Immunity 30(3):397-407(2009) McKinnon, E., et al. Diabetes Obes Metab 11 SUPPL 1, 92-100 (2009) : Bromberg, K.D., et al. Cancer Res. 67(17):8172-8179(2007)
