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RNF5 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - RNF5 Antibody (Center) AP12440c
    Western blot analysis of lysate from K562 cell line, using RNF5 Antibody (Center)(Cat. #AP12440c). AP12440c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
  • IHC-P - RNF5 Antibody (Center) AP12440c
    RNF5 Antibody (Center) (Cat. #AP12440c)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RNF5 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q99942
Other Accession Q5M807, O35445, NP_008844.1
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 19881 Da
Antigen Region 65-94 aa
Additional Information
Gene ID 6048
Other Names E3 ubiquitin-protein ligase RNF5, 632-, Protein G16, RING finger protein 5, Ram1 homolog, HsRma1, RNF5, G16, NG2, RMA1
Target/Specificity This RNF5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 65-94 amino acids from the Central region of human RNF5.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRNF5 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RNF5
Synonyms G16, NG2, RMA1
Function Has E2-dependent E3 ubiquitin-protein ligase activity. May function together with E2 ubiquitin-conjugating enzymes UBE2D1/UBCH5A and UBE2D2/UBC4. Mediates ubiquitination of PXN/paxillin and Salmonella type III secreted protein sopA. May be involved in regulation of cell motility and localization of PXN/paxillin. Mediates the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD; the ubiquitination appears to involve E2 ubiquitin-conjugating enzyme UBE2N. Mediates the 'Lys-48'-linked polyubiquitination of TMEM173 at 'Lys-150' leading to its proteasomal degradation; the ubiquitination occurrs in mitochondria after viral transfection and regulates antiviral responses.
Cellular Location Membrane; Multi-pass membrane protein. Mitochondrion membrane. Endoplasmic reticulum membrane Note=Predominantly located in the plasma membrane, with some localization occurring within cytoplasmic organelles
Tissue Location Widely expressed.
Research Areas
Citations ( 0 )


The protein encoded by this gene contains a RING finger, which is a motif known to be involved in protein-protein interactions. This protein is a membrane-bound ubiquitin ligase. It can regulate cell motility by targeting paxillin ubiquitination and altering the distribution and localization of paxillin in cytoplasm and cell focal adhesions.


Barcellos, L.F., et al. PLoS Genet. 5 (10), E1000696 (2009) :
Tcherpakov, M., et al. J. Biol. Chem. 284(18):12099-12109(2009)
Zhong, B., et al. Immunity 30(3):397-407(2009)
McKinnon, E., et al. Diabetes Obes Metab 11 SUPPL 1, 92-100 (2009) :
Bromberg, K.D., et al. Cancer Res. 67(17):8172-8179(2007)

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$ 295.00
$ 99.00
Cat# AP12440c
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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