Register or Login
All
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   RASSF10 Antibody (Center)   

RASSF10 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - RASSF10 Antibody (Center) AP12444c
    Anti-RASSF10 Antibody (Center) at 1:500 dilution + human lung lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - RASSF10 Antibody (Center) AP12444c
    RASSF10 Antibody (Center) (Cat. #AP12444c) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the RASSF10 antibody detected the RASSF10 protein (arrow).
  • IHC-P - RASSF10 Antibody (Center) AP12444c
    RASSF10 Antibody (Center) (Cat. #AP12444c)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RASSF10 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • SPECIFICATION
  • CITATIONS: 1
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession A6NK89
Other Accession NP_001073990.2
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Antigen Region 230-259 aa
Additional Information
Other Names Ras association domain-containing protein 10, RASSF10
Target/Specificity This RASSF10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 230-259 amino acids from the Central region of human RASSF10.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRASSF10 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RASSF10
Cellular Location Cytoplasm, cytosol. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Note=During interphase, predominantly cytoplasmic, although some nuclear staining in several tumor cell contexts. During prophase, observed at developing centrosomes. Displays persistent localization with centrosomally radiating microtubule bundles until late telophase Associates with spindle poles particularly during metaphase and anaphase before relocating back to the cytoplasm
Tissue Location Expressed in brain. Tends to be down-regulated in astrocytic gliomas due to promoter methylation. Methylation occurs early in gliomagenesis and the extent of methylation parallels with higher glioma grades, so that methylation is observed in close to 70% WHO grade IV primary glioblastomas, but not in grade I astrocytomas. EMBL; AC084859; -; NOT_ANNOTATED_CDS; Genomic_DNA RefSeq; NP_001073990.2; NM_001080521.2 UniGene; Hs.134650; - ProteinModelPortal; A6NK89; - SMR; A6NK89; - BioGrid; 570026; 38 IntAct; A6NK89; 61 iPTMnet; A6NK89; - PhosphoSitePlus; A6NK89; - BioMuta; RASSF10; - PRIDE; A6NK89; - ProteomicsDB; 1387; - Ensembl; ENST00000529419; ENSP00000485526; ENSG00000189431 GeneID; 644943; - KEGG; hsa:644943; - UCSC; uc021qdz.2; human CTD; 644943; - DisGeNET; 644943; - EuPathDB; HostDB:ENSG00000189431.7; - GeneCards; RASSF10; - HGNC; HGNC:33984; RASSF10 HPA; HPA040406; - MIM; 614713; gene neXtProt; NX_A6NK89; - OpenTargets; ENSG00000189431; - GeneTree; ENSGT00510000047164; - HOGENOM; HOG000065674; - HOVERGEN; HBG053178; - InParanoid; A6NK89; - KO; K09856; - OMA; DLDYSQQ; - OrthoDB; EOG091G09E9; - PhylomeDB; A6NK89; - GenomeRNAi; 644943; - PRO; PR:A6NK89; - Proteomes; UP000005640; Chromosome 11 Bgee; ENSG00000189431; - CleanEx; HS_RASSF10; - Genevisible; A6NK89; HS GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell GO; GO:0005815; C:microtubule organizing center; IEA:UniProtKB-SubCell GO; GO:0000922; C:spindle pole; IEA:UniProtKB-SubCell GO; GO:0007165; P:signal transduction; IEA:InterPro InterPro; IPR033593; N-RASSF InterPro; IPR000159; RA_dom InterPro; IPR033634; RASSF10 InterPro; IPR029071; Ubiquitin-like_domsf PANTHER; PTHR15286; PTHR15286; 1 PANTHER; PTHR15286:SF13; PTHR15286:SF13; 1 SUPFAM; SSF54236; SSF54236; 2 PROSITE; PS50200; RA; 1 2: Evidence at transcript level; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Polymorphism; Reference proteome CHAIN 1 507 Ras association domain-containing protein 10 /FTId=PRO_0000332284 DOMAIN 4 133 Ras-associating. {ECO:0000255|PROSITE- ProRule:PRU00166} COILED 237 264 COILED 319 358 COMPBIAS 197 221 Ser-rich VARIANT 194 194 Q -> H (in dbSNP:rs4323847) /FTId=VAR_042999 SEQUENCE 507 AA; 56900 MW; A9A5D0A546F61518 CRC64; MDPSEKKISV WICQEEKLVS GLSRRTTCSD VVRVLLEDGC RRRRRQRRSR RLGSAGDPHG PGELPEPPNE DDEDDDEALP QGMLCGPPQC YCIVEKWRGF ERILPNKTRI LRLWAAWGEE QENVRFVLVR SEASLPNAGP RSAEARVVLS RERPCPARGA PARPSLAMTQ EKQRRVVRKA FRKLAKLNRR RQQQTPSSCS STSSSTASSC SSSPRTHESA SVERMETLVH LVLSQDHTIR QQVQRLHELD REIDHYEAKV HLDRMRRHGV NYVQDTYLVG AGIELDGSRP GEEPEEVAAE AEEAAAAPPL AGEAQAAALE ELARRCDDLL RLQEQRVQQE ELLERLSAEI QEELNQRWMR RRQEELAARE EPLEPDGGPD GELLLEQERV RTQLSTSLYI GLRLNTDLEA VKSDLDYSQQ QWDSKKRELQ GLLQTLHTLE LTVAPDGAPG SGSPSREPGP QACADMWVDQ ARGLAKSGPG NDEDSDTGLS SMHSQDSDSL PMCESLV
Research Areas
Citations ( 0 )

References

Schagdarsurengin, U., et al. Epigenetics 4(8):571-576(2009)
Hesson, L.B., et al. Mol. Cancer 8, 42 (2009) :
Sherwood, V., et al. Mol. Biol. Cell 19(4):1772-1782(2008)

FeedBack
Abgent welcomes feedback from its customers.

If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at tech@abgent.com.

$ 295.00
$ 99.00
Cat# AP12444c
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
Request Quote Here

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaCentral AmericaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSouth KoreaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamWorldwideOthers
Abgent, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609
USA Headquarters
(888) 735-7227 / (858) 622-0099 or (858) 875-1900

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
“Crown Flash”  50 % off on 2,500+ Crown Antibodies. PromoCode:<span class=text-red> FLASH50
Terms & Conditions