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RASSF10 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - RASSF10 Antibody (Center) AP12444c
    Anti-RASSF10 Antibody (Center) at 1:500 dilution + human lung lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - RASSF10 Antibody (Center) AP12444c
    RASSF10 Antibody (Center) (Cat. #AP12444c) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the RASSF10 antibody detected the RASSF10 protein (arrow).
  • IHC-P - RASSF10 Antibody (Center) AP12444c
    RASSF10 Antibody (Center) (Cat. #AP12444c)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RASSF10 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession A6NK89
Other Accession NP_001073990.2
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 56900 Da
Antigen Region 230-259 aa
Additional Information
Gene ID 644943
Other Names Ras association domain-containing protein 10, RASSF10
Target/Specificity This RASSF10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 230-259 amino acids from the Central region of human RASSF10.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRASSF10 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RASSF10
Cellular Location Cytoplasm, cytosol. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Note=During interphase, predominantly cytoplasmic, although some nuclear staining in several tumor cell contexts. During prophase, observed at developing centrosomes. Displays persistent localization with centrosomally radiating microtubule bundles until late telophase Associates with spindle poles particularly during metaphase and anaphase before relocating back to the cytoplasm
Tissue Location Expressed in brain. Tends to be down-regulated in astrocytic gliomas due to promoter methylation. Methylation occurs early in gliomagenesis and the extent of methylation parallels with higher glioma grades, so that methylation is observed in close to 70% WHO grade IV primary glioblastomas, but not in grade I astrocytomas.
Research Areas
Citations ( 0 )


Schagdarsurengin, U., et al. Epigenetics 4(8):571-576(2009)
Hesson, L.B., et al. Mol. Cancer 8, 42 (2009) :
Sherwood, V., et al. Mol. Biol. Cell 19(4):1772-1782(2008)

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$ 295.00
$ 99.00
Cat# AP12444c
(40 western blots)
Availability: In Stock
Bulk Size
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