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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   PIAS3 Antibody (C-term)   

PIAS3 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • IHC-P - PIAS3 Antibody (C-term) AP1244a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • WB - PIAS3 Antibody (C-term) AP1244a
    Western blot analysis of PIAS3 Antibody (C-term) (Cat. #AP1244a) in PIAS3 cell line lysate (35ug/lane). PIAS3 (arrow) was detected using the purified Pab.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9Y6X2
Other Accession O70260, O54714
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 68017 Da
Antigen Region 588-619 aa
Additional Information
Gene ID 10401
Other Names E3 SUMO-protein ligase PIAS3, 632-, Protein inhibitor of activated STAT protein 3, PIAS3
Target/Specificity This PIAS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 588-619 amino acids of human PIAS3.
Dilution IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPIAS3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PIAS3
Function Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation.
Cellular Location Cytoplasm. Nucleus. Note=Mainly cytoplasmic but shuttles between the nucleus and cytoplasm. Transported to the nucleus on interaction with phosphorylated STAT3. Colocalizes with GFI1 in nuclear dots (By similarity).
Tissue Location Isoform 1 is expressed in most tissues except thymus and small intestine. Isoform 3 is expressed only in brain, heart, thymus, muscle, lung, testis, lactating breast and embryonic stem cells.
Research Areas
Citations ( 0 )


PIAS3 is a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription)] family of transcriptional modulators. The protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor, catalyzing the covalent attachment of a SUMO protein to specific target substrates. PIAS3 plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context.


Nojiri, S., et al., Biochem. Biophys. Res. Commun. 314(1):97-103 (2004). Long, J., et al., Proc. Natl. Acad. Sci. U.S.A. 101(1):99-104 (2004). Cheng, J., et al., Leuk. Res. 28(1):71-82 (2004). Yamamoto, T., et al., Biochem. Biophys. Res. Commun. 306(2):610-615 (2003). Ueki, N., et al., J. Hum. Genet. 44(3):193-196 (1999).

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$ 295.00
$ 99.00
Cat# AP1244a
(40 western blots)
Availability: In Stock
Bulk Size
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