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>   home   >   Products   >   Primary Antibodies   >   POLR2G Antibody (C-term)   

POLR2G Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - POLR2G Antibody (C-term) AP12458b
    POLR2G Antibody (C-term) (Cat. #AP12458b) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the POLR2G antibody detected the POLR2G protein (arrow).
  • FC - POLR2G Antibody (C-term) AP12458b
    POLR2G Antibody (C-term) (Cat. #AP12458b) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P62487
Other Accession P62489, P62488, Q7ZW41, Q5E9B8, NP_002687.1
Reactivity Human
Predicted Bovine, Zebrafish, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 19294 Da
Antigen Region 126-153 aa
Additional Information
Gene ID 5436
Other Names DNA-directed RNA polymerase II subunit RPB7, RNA polymerase II subunit B7, DNA-directed RNA polymerase II subunit G, RNA polymerase II 19 kDa subunit, RPB19, POLR2G, RPB7
Target/Specificity This POLR2G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 126-153 amino acids from the C-terminal region of human POLR2G.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPOLR2G Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms RPB7
Function DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB7 is part of a subcomplex with RPB4 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA (By similarity). Binds RNA.
Cellular Location Nucleus.
Citations (0)

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This gene encodes the seventh largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The protein functions in transcription initiation, and is also thought to help stabilize transcribing polyermase molecules during elongation.


Cojocaru, M., et al. Biochem. J. 409(1):139-147(2008)
Ujvari, A., et al. Nat. Struct. Mol. Biol. 13(1):49-54(2006)
Meka, H., et al. Nucleic Acids Res. 33(19):6435-6444(2005)
Lehner, B., et al. Genomics 83(1):153-167(2004)
Zhou, M., et al. Proc. Natl. Acad. Sci. U.S.A. 100(22):12666-12671(2003)

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$ 295.00
$ 99.00
Cat# AP12458b
(40 western blots)
Availability: In Stock
Bulk Size
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