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PIAS3 Antibody (N-term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP1245a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 1
- Reviews
- Protocols
- Backgrounds
PIAS3 Antibody (N-term) - Product info | |
| Application | WB, IHC
|
| Primary Accession | Q9Y6X2 |
| Other Accession | O70260, O54714 |
| Reactivity | Human |
| Predicted | Mouse, Rat |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 68017 Da |
PIAS3 Antibody (N-term) - Additional info | |
| Gene ID 10401 | |
| Other Names PIAS3; E3 SUMO-protein ligase PIAS3; Protein inhibitor of activated STAT protein 3 | |
| Target/Specificity This PIAS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 110~140 amino acids from the N-terminal region of human PIAS3. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:50~100 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions PIAS3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
PIAS3 Antibody (N-term) - Protein Information | |
| Name PIAS3 | |
| Function Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth | |
| Cellular Location Cytoplasm. Nucleus. Note=Mainly cytoplasmic but shuttles between the nucleus and cytoplasm. Transported to the nucleus on interaction with phosphorylated STAT3. Colocalizes with GFI1 in nuclear dots (By similarity) | |
| Tissue Location Isoform 1 is expressed in most tissues except thymus and small intestine. Isoform 3 is expressed only in brain, heart, thymus, muscle, lung, testis, lactating breast and embryonic stem cells | |
PIAS3 Antibody (N-term) - Related products
AP1244a: PIAS3 Antibody (C-term)
AP1245a: PIAS3 Antibody (N-term)
RI13979: PIAS3 predesign siRNA
PIAS3 Antibody (N-term) - Application data
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Western blot analysis of anti-PIAS3 Pab (Cat. #AP1245a) in HL60 cell line lysate (35ug/lane). PIAS3(arrow) was detected using the purified Pab.
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
PIAS3 Antibody (N-term) - Research Areas
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BACKGROUND
PIAS3 is a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription)] family of transcriptional modulators. The protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor, catalyzing the covalent attachment of a SUMO protein to specific target substrates. PIAS3 plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context.
REFERENCES
Nojiri, S., et al., Biochem. Biophys. Res. Commun. 314(1):97-103 (2004). Long, J., et al., Proc. Natl. Acad. Sci. U.S.A. 101(1):99-104 (2004). Cheng, J., et al., Leuk. Res. 28(1):71-82 (2004). Yamamoto, T., et al., Biochem. Biophys. Res. Commun. 306(2):610-615 (2003). Ueki, N., et al., J. Hum. Genet. 44(3):193-196 (1999).
