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SH3D20 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - SH3D20 Antibody (Center) AP12523c
    All lanes : Anti-SH3D20 Antibody (Center) at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 98 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q6ZUM4
Other Accession NP_001152802.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 98396 Da
Antigen Region 198-226 aa
Additional Information
Gene ID 201176
Other Names Rho GTPase-activating protein 27, CIN85-associated multi-domain-containing Rho GTPase-activating protein 1, Rho-type GTPase-activating protein 27, SH3 domain-containing protein 20, ARHGAP27, CAMGAP1, SH3D20
Target/Specificity This SH3D20 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 198-226 amino acids from the Central region of human SH3D20.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSH3D20 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ARHGAP27
Synonyms CAMGAP1, SH3D20
Function Rho GTPase-activating protein which may be involved in clathrin-mediated endocytosis. GTPase activators for the Rho-type GTPases act by converting them to an inactive GDP-bound state. Has activity toward CDC42 and RAC1 (By similarity).
Cellular Location Cytoplasm. Membrane; Peripheral membrane protein
Tissue Location Expressed in germinal center B-cell, spleen, chronic lymphocytic leukemia, pancreatic cancer and lung cancer
Research Areas
Citations (0)

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Background

SH3D20 is a rho GTPase-activating protein which may be involved in clathrin-mediated endocytosis. GTPase activators for the Rho-type GTPases act by converting them to an inactive GDP-bound state. Has activity toward CDC42 and RAC1 (By similarity).

References

Edwards, T.L., et al. Ann. Hum. Genet. 74(2):97-109(2010)

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$ 295.00
$ 99.00
Cat# AP12523c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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