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GAA Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • IHC - GAA Antibody (N-term) AP12544a
    AP12544a staining GAA in Human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • WB - GAA Antibody (N-term) AP12544a
    Western blot analysis of lysate from Jurkat cell line, using GAA Antibody (N-term)(Cat. #AP12544a). AP12544a was diluted at 1:2000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
  • WB - GAA Antibody (N-term) AP12544a
    Western blot analysis of lysates from A549, MCF-7, SW620, PC-3 cell line (from left to right), using GAA Antibody (N-term)(Cat. #AP12544a). AP12544a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
  • WB - GAA Antibody (N-term) AP12544a
    GAA Antibody (N-term) (Cat. #AP12544a) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the GAA antibody detected the GAA protein (arrow).
  • IHC-P - GAA Antibody (N-term) AP12544a
    GAA Antibody (N-term) (Cat. #AP12544a)immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GAA Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P10253
Other Accession NP_000143.2, NP_001073271.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 105324 Da
Antigen Region 174-203 aa
Additional Information
Gene ID 2548
Other Names Lysosomal alpha-glucosidase, Acid maltase, Aglucosidase alfa, 76 kDa lysosomal alpha-glucosidase, 70 kDa lysosomal alpha-glucosidase, GAA
Target/Specificity This GAA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 174-203 amino acids from the N-terminal region of human GAA.
Dilution IHC~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGAA Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GAA
Function Essential for the degradation of glygogen to glucose in lysosomes.
Cellular Location Lysosome. Lysosome membrane
Research Areas
Citations (0)

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This gene encodes acid alpha-glucosidase, which is essential for the degradation of glycogen to glucose in lysosomes. Different forms of acid alpha-glucosidase are obtained by proteolytic processing. Defects in this gene are the cause of glycogen storage disease II, also known as Pompe's disease, which is an autosomal recessive disorder with a broad clinical spectrum. Three transcript variants encoding the same protein have been found for this gene.


Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Labrousse, P., et al. Mol. Genet. Metab. 99(4):379-383(2010)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Aoyama, Y., et al. J. Hum. Genet. 54(11):681-686(2009)
Maimaiti, M., et al. J. Hum. Genet. 54(8):493-496(2009)

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$ 295.00
$ 99.00
Cat# AP12544a
(40 western blots)
Availability: In Stock
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