|Application ||WB, E|
|Other Accession||Q4R3I8, NP_002910.1, NP_602304.1|
|Calculated MW||83530 Da|
|Antigen Region||661-690 aa|
|Other Names||Transcription factor RFX3, Regulatory factor X 3, RFX3|
|Target/Specificity||This RFX3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 661-690 amino acids from the C-terminal region of human RFX3.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RFX3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcription factor required for ciliogenesis and islet cell differentiation during endocrine pancreas development. Essential for the differentiation of nodal monocilia and left- right asymmetry specification during embryogenesis. Required for the biogenesis of motile cilia by governing growth and beating efficiency of motile cells. Also required for ciliated ependymal cell differentiation. Regulates the expression of genes involved in ciliary assembly (DYNC2LI1, FOXJ1 and BBS4) and genes involved in ciliary motility (DNAH11, DNAH9 and DNAH5) (By similarity). Together with RFX6, participates in the differentiation of 4 of the 5 islet cell types during endocrine pancreas development, with the exception of pancreatic PP (polypeptide-producing) cells. Regulates transcription by forming a heterodimer with another RFX protein and binding to the X-box in the promoter of target genes (PubMed:20148032). Represses transcription of MAP1A in non- neuronal cells but not in neuronal cells (PubMed:12411430).|
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Provided below are standard protocols that you may find useful for product applications.
This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X1, X2, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with other RFX family members. Two transcript variants encoding different isoforms have been described for this gene, and at least one of the variants utilizes alternative polyadenylation signals.
El Zein, L., et al. J. Cell. Sci. 122 (PT 17), 3180-3189 (2009) :
Humphray, S.J., et al. Nature 429(6990):369-374(2004)
Maijgren, S., et al. Arch. Dermatol. Res. 295(11):482-489(2004)
Nakayama, A., et al. J. Biol. Chem. 278(1):233-240(2003)
Morotomi-Yano, K., et al. J. Biol. Chem. 277(1):836-842(2002)
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