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MED8 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - MED8 Antibody (Center) AP12649c
    MED8 Antibody (Center) (Cat. #AP12649c) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the MED8 antibody detected the MED8 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q96G25
Other Accession Q9D7W5, Q6NYT1, NP_443109.2, NP_963836.2
Reactivity Human
Predicted Zebrafish, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 29080 Da
Antigen Region 95-122 aa
Additional Information
Gene ID 112950
Other Names Mediator of RNA polymerase II transcription subunit 8, Activator-recruited cofactor 32 kDa component, ARC32, Mediator complex subunit 8, MED8
Target/Specificity This MED8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-122 amino acids from the Central region of human MED8.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMED8 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MED8
Function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May play a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins.
Cellular Location Nucleus.
Citations (0)

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This gene encodes a protein that is one of more than 20 subunits of the mediator complex, first identified in S. cerevisiae, that is required for activation of transcription. The product of this gene also interacts with elongins B and C, and CUL2 and RBX1, to reconstitute a ubiquitin ligase. Two alternative transcripts encoding different isoforms have been described.


Leonard, V.H., et al. J. Virol. 80(19):9667-9675(2006)
Wang, A.G., et al. Biochem. Biophys. Res. Commun. 345(3):1022-1032(2006)
Sato, S., et al. Mol. Cell 14(5):685-691(2004)
Tomomori-Sato, C., et al. J. Biol. Chem. 279(7):5846-5851(2004)
Sato, S., et al. J. Biol. Chem. 278(50):49671-49674(2003)

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$ 295.00
$ 99.00
Cat# AP12649c
(40 western blots)
Availability: In Stock
Bulk Size
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