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>   home   >   Products   >   Primary Antibodies   >   Metabolism   >   IREB2 Antibody (Center)   

IREB2 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - IREB2 Antibody (Center) AP12657C
    IREB2 Antibody (Center) (Cat. #AP12657c) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the IREB2 antibody detected the IREB2 protein (arrow).
  • IHC-P - IREB2 Antibody (Center) AP12657C
    IREB2 Antibody (Center) (Cat. #AP12657c)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IREB2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - IREB2 Antibody (Center) AP12657C
    IREB2 Antibody (Center) (Cat. #AP12657c) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession P48200
Other Accession Q62751, B3VKQ2, Q811J3, NP_004127.1
Reactivity Human
Predicted Mouse, Pig, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 105045 Da
Antigen Region 201-230 aa
Additional Information
Gene ID 3658
Other Names Iron-responsive element-binding protein 2, IRE-BP 2, Iron regulatory protein 2, IRP2, IREB2
Target/Specificity This IREB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 201-230 amino acids from the Central region of human IREB2.
Dilution WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIREB2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IREB2
Function RNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'- UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA.
Cellular Location Cytoplasm.
Research Areas
Citations (0)

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Background

IREB2 is a RNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA.

References

Ucisik-Akkaya, E., et al. Mol. Hum. Reprod. 16(10):770-777(2010)
Hansen, H.M., et al. Hum. Mol. Genet. 19(18):3652-3661(2010)
Cho, M.H., et al. Nat. Genet. 42(3):200-202(2010)
Salahudeen, A.A., et al. Science 326(5953):722-726(2009)
DeMeo, D.L., et al. Am. J. Hum. Genet. 85(4):493-502(2009)

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$ 295.00
$ 99.00
Cat# AP12657C
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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