|Application ||WB, IHC-P, E|
|Other Accession||NP_001019386.1, NP_060026.2|
|Calculated MW||40670 Da|
|Antigen Region||294-323 aa|
|Other Names||Filamin-binding LIM protein 1, FBLP-1, Migfilin, Mitogen-inducible 2-interacting protein, MIG2-interacting protein, FBLIM1, FBLP1|
|Target/Specificity||This FBLIM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 294-323 amino acids from the C-terminal region of human FBLIM1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||FBLIM1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Serves as an anchoring site for cell-ECM adhesion proteins and filamin-containing actin filaments. Is implicated in cell shape modulation (spreading) and motility. May participate in the regulation of filamin-mediated cross-linking and stabilization of actin filaments. May also regulate the assembly of filamin- containing signaling complexes that control actin assembly. Promotes dissociation of FLNA from ITGB3 and ITGB7. Promotes activation of integrins and regulates integrin-mediated cell-cell adhesion.|
|Cellular Location||Cytoplasm, cell cortex. Cell junction, focal adhesion. Cytoplasm, cytoskeleton. Note=Associated with actin stress fiber at cell-ECM focal adhesion sites. Isoform 1 and isoform 3 are recruited and localized at actin stress fibers and clustered at cell-EMC adhesion sites through interaction with FERMT2. Isoform 2 is localized at actin stress fibers|
|Tissue Location||Isoform 1 and isoform 3 are expressed in heart, kidney, lung, pancreas, placenta and platelets. Isoform 2 is expressed in brain, heart, kidney, lung, pancreas, placenta, skeletal muscle and platelets.|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a protein with an N-terminal filamin-binding domain, a central proline-rich domain, and, multiple C-terminal LIM domains. This protein localizes at cell junctions and may link cell adhesion structures to the actin cytoskeleton. This protein may be involved in the assembly and stabilization of actin-filaments and likely plays a role in modulating cell adhesion, cell morphology and cell motility. This protein also localizes to the nucleus and may affect cardiomyocyte differentiation after binding with the CSX/NKX2-5 transcription factor. Alternative splicing results in multiple transcript variants encoding different isoforms.
Zhao, J., et al. J. Biol. Chem. 284(49):34308-34320(2009)
Ithychanda, S.S., et al. J. Biol. Chem. 284(7):4713-4722(2009)
Lad, Y., et al. J. Biol. Chem. 283(50):35154-35163(2008)
Lai-Cheong, J.E., et al. J. Invest. Dermatol. 128(9):2156-2165(2008)
Papachristou, D.J., et al. Histopathology 51(4):499-508(2007)
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