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ZP4 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - ZP4 Antibody (C-term) AP12724b
    Anti-ZP4 Antibody (C-term) at 1:2000 dilution + NCCIT whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 59 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - ZP4 Antibody (C-term) AP12724b
    ZP4 Antibody (C-term) (Cat. #AP12724b) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the ZP4 antibody detected the ZP4 protein (arrow).
  • IHC-P - ZP4 Antibody (C-term) AP12724b
    ZP4 Antibdy (C-term) (Cat. #AP12724b)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ZP4 Antibdy (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q12836
Other Accession NP_067009.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 59400 Da
Antigen Region 445-474 aa
Additional Information
Gene ID 57829
Other Names Zona pellucida sperm-binding protein 4, Zona pellucida glycoprotein 4, Zp-4, Zona pellucida protein B, Processed zona pellucida sperm-binding protein 4, ZP4, ZPB
Target/Specificity This ZP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 445-474 amino acids from the C-terminal region of human ZP4.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsZP4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ZP4
Synonyms ZPB
Function The mammalian zona pellucida, which mediates species- specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP4 may act as a sperm receptor.
Cellular Location Processed zona pellucida sperm-binding protein 4: Secreted, extracellular space, extracellular matrix {ECO:0000250|UniProtKB:Q00193}. Note=The glycoproteinaceous translucent extracellular matrix that surrounds the mammalian oocyte is called zona pellucida.
Tissue Location Oocytes.
Research Areas
Citations ( 0 )


The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a consensus furin cleavage site, and a C-terminal transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. Previously, this gene has been referred to as ZP1 or ZPB and thought to have similar functions as mouse Zp1. However, a human gene with higher similarity and chromosomal synteny to mouse Zp1 has been assigned the symbol ZP1 and this gene has been assigned the symbol ZP4.


Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
McCauley, J.L., et al. Genes Immun. 10(7):624-630(2009)
Nakano, M., et al. Proc. Natl. Acad. Sci. U.S.A. 106(31):12838-12842(2009)
Choudhury, S., et al. J. Reprod. Immunol. 79(2):137-147(2009)
Chiu, P.C., et al. Biol. Reprod. 79(5):869-877(2008)

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$ 295.00
$ 99.00
Cat# AP12724b
(40 western blots)
Availability: In Stock
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