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SIX5 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IF, IHC-P, WB, E |
|---|---|
| Primary Accession | Q8N196 |
| Other Accession | P70178, NP_787071.2 |
| Reactivity | Human |
| Predicted | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 74562 Da |
| Antigen Region | 257-286 aa |
| Gene ID | 147912 |
|---|---|
| Other Names | Homeobox protein SIX5, DM locus-associated homeodomain protein, Sine oculis homeobox homolog 5, SIX5, DMAHP |
| Target/Specificity | This SIX5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 257-286 amino acids from the Central region of human SIX5. |
| Dilution | IF~~1:10~50 WB~~1:1000 IHC-P~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | SIX5 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SIX5 |
|---|---|
| Synonyms | DMAHP |
| Function | Transcription factor that is thought to be involved in regulation of organogenesis. May be involved in determination and maintenance of retina formation. Binds a 5'-GGTGTCAG-3' motif present in the ARE regulatory element of ATP1A1. Binds a 5'-TCA[AG][AG]TTNC-3' motif present in the MEF3 element in the myogenin promoter, and in the IGFBP5 promoter (By similarity). Thought to be regulated by association with Dach and Eya proteins, and seems to be coactivated by EYA1, EYA2 and EYA3 (By similarity). |
| Cellular Location | Cytoplasm. Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108, ECO:0000269|PubMed:12500905} |
| Tissue Location | Expressed in adult but not in fetal eyes. Found in corneal epithelium and endothelium, lens epithelium, ciliary body epithelia, cellular layers of the retina and the sclera |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded by this gene is a homeodomain-containing transcription factor that appears to function in the regulation of organogenesis. This gene is located downstream of the dystrophia myotonica-protein kinase gene. Mutations in this gene are a cause of branchiootorenal syndrome type 2.
References
Yoshida, T., et al. Int. J. Mol. Med. 25(4):649-656(2010)
Oguri, M., et al. Am. J. Hypertens. 23(1):70-77(2010)
Hoskins, B.E., et al. Am. J. Hum. Genet. 80(4):800-804(2007)
Sato, S., et al. Hum. Mol. Genet. 11(9):1045-1058(2002)
Fougerousse, F., et al. J. Muscle Res. Cell. Motil. 23(3):255-264(2002)
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