|Application ||WB, IHC-P, E|
|Other Accession||NP_001002019.1, NP_079491.2|
|Calculated MW||47470 Da|
|Antigen Region||322-350 aa|
|Other Names||tRNA pseudouridine synthase A, mitochondrial, tRNA pseudouridine(38-40) synthase, tRNA pseudouridylate synthase I, tRNA-uridine isomerase I, PUS1|
|Target/Specificity||This PUS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 322-350 amino acids from the C-terminal region of human PUS1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PUS1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Converts specific uridines to PSI in a number of tRNA substrates. Acts on positions 27/28 in the anticodon stem and also positions 34 and 36 in the anticodon of an intron containing tRNA. Involved in regulation of nuclear receptor activity possibly through pseudouridylation of SRA1 RNA (By similarity).|
|Cellular Location||Isoform 1: Mitochondrion|
|Tissue Location||Widely expressed. High levels of expression found in brain and skeletal muscle.|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a pseudouridine synthase that converts uridine to pseudouridine once it has been incorporated into an RNA molecule. The encoded enzyme may play an essential role in tRNA function and in stabilizing the secondary and tertiary structure of many RNAs. A mutation in this gene has been linked to mitochondrial myopathy and sideroblastic anemia. Alternate splicing results in multiple transcript variants.
Bergmann, A.K., et al. Pediatr Blood Cancer 54(2):273-278(2010)
Sibert, B.S., et al. RNA 14(9):1895-1906(2008)
Rikova, K., et al. Cell 131(6):1190-1203(2007)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Fernandez-Vizarra, E., et al. J. Med. Genet. 44(3):173-180(2007)
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