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PHACTR3 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PHACTR3 Antibody (N-term) AP12836A
    PHACTR3 Antibody (N-term) (Cat. #AP12836a) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the PHACTR3 antibody detected the PHACTR3 protein (arrow).
  • IHC-P - PHACTR3 Antibody (N-term) AP12836A
    PHACTR3 Antibody (N-term) (Cat. #AP12836a)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PHACTR3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q96KR7
Other Accession NP_899067.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 62552 Da
Antigen Region 17-46 aa
Additional Information
Gene ID 116154
Other Names Phosphatase and actin regulator 3, Scaffold-associated PP1-inhibiting protein, Scapinin, PHACTR3, C20orf101, SCAPIN1
Target/Specificity This PHACTR3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-46 amino acids from the N-terminal region of human PHACTR3.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPHACTR3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PHACTR3
Synonyms C20orf101, SCAPIN1
Cellular Location Nucleus matrix. Note=Localized to the nuclear matrix-intermediate filament scaffold. Isoform 2 is also found in some cytoplasmic extensions
Tissue Location Abundantly expressed in brain. Also found in several tumors such as lung carcinomas, nervous tumors and HL-60 leukemia cells. Isoform 3 is the major form in U-937, GOTO and HL- 60 leukemia cells
Research Areas
Citations (0)

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Background

The protein encoded by this gene is associated with the nuclear scaffold in proliferating cells. It was found to bind to the catalytic subunit of protein phosphatase-1 (PP1) and inhibit PP1 activity, suggesting that this protein may function as a regulatory subunit of PP1. Alternative splicing at this locus results in several transcript variants encoding different isoforms.

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Bankovic, J., et al. Lung Cancer 67(2):151-159(2010)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Sagara, J., et al. PLoS ONE 4 (1), E4247 (2009) :

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Cat# AP12836A
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(40 western blots)
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